| HPLC法测定面条树中的鸭脚树叶碱 |
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| 引用本文： | 梁聂彦,陈学松. HPLC法测定面条树中的鸭脚树叶碱[J]. 中国药师, 2012, 15(4): 495-497 |
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| 作者姓名： | 梁聂彦 陈学松 |
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| 作者单位： | 1. 广西壮族自治区岑溪市人民医院,广西梧州,543200
2. 广西壮族自治区梧州食品药品检验所 |
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| 摘 要： | 目的:建立面条树中鸭脚树叶碱的HPLC含量测定法.方法:色谱柱为Thermo Gold C18(250 mm×4.60 mm,5 μm),检测波长为287 nm,流动相为甲醇-水-三乙胺(47:53:0.005,V/V),流速为1.0 ml·min-1,柱温为35℃.结果:鸭脚树叶碱在0.14～14.00 μg范围内呈良好的线性关系,r=0.999 9;平均回收率为96.98%,RSD=0.9%(n=9).结论:该法简便、准确,可用于面条树的质量控制研究.
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| 关 键 词： | 面条树 高效液相色谱法 鸭脚树叶碱 含量测定 |
| 收稿时间： | 2011-11-09 |
| 修稿时间： | 2012-01-31 |
Determination of Picrinine in Alstonia scholaris by HPLC |
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| Liang Nieyan and Chen Xuesong. Determination of Picrinine in Alstonia scholaris by HPLC[J]. China Pharmacist, 2012, 15(4): 495-497 |
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| Authors: | Liang Nieyan and Chen Xuesong |
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| Affiliation: | 1.Cenxi People＇s Hospital of Guangxi Zhuang Autonomous Region,Guangxi Wuzhou 543200,China;2.Wuzhou Institute for Food and Drug Control of Guangxi Zhuang Autonomous Region) |
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| Abstract: | Objective： To establish an HPLC method for the determination of picrinine in Alstonia scholaris.Method： A Thermo Gold C18（250 mm×4.60 mm,5 μm）column was used,the detection wavelength was 287 nm,the mobile phase was methanol-water-triethylamine（47∶ 53∶ 0.005,V/V）,the flow rate was 1.0 ml·min-1 and the column temperature was 35℃.Result： The linear range of picrinine was within 0.14-14.00 μg（r=0.999 9）,and the average recovery was 96.98% with RSD of 0.9%（n= 9）.Conclusion： The method is simple and accurate,and it can be used for the quality control of Alstonia scholaris. |
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| Keywords: | Alstonia scholaris HPLC Picrinine Determination |
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