气相色谱法测定风油精中5种成分的含量

目的研究提高风油精中薄荷脑、桉油精、樟脑、丁香酚、水杨酸甲酯5种成分的含量测定方法。方法色谱柱：Agilent INNOWAX石英毛细管柱（30m×0.32mm×0.25μm）;载气为氮气;柱温采用程序升温：初始温度85℃,保持4min,以每分钟30℃的速率升至115℃,保持0min,以每分钟70℃的速率升至200℃,保持4min,以每分钟100℃的速率升至250℃,保持3min;检测器：FID;采用内标法测定。结果薄荷脑、桉油精、樟脑、水杨酸甲酯、丁香酚线性范围为0.34～17.22mg.mL-1（r=1.0000）、0.05～2.26mg.mL-1（r=1.0000）,0.04～1.93mg.mL-1（r=0.9999）,0.34～17.16mg.mL-1（r=1.0000）、0.04～1.80mg.mL-1（r=1.0000）,平均回收率为96.17%、96.69%、101.14%、96.86%、98.02%。结论该方法经济、简单、准确且重现性好,可作为风油精的含量测定质量控制标准。

Simultaneous determination of five compositions in Fengyoujing by GC

OBJECTIVE To establish a GC method for simultaneous determination of Menthol,Cineol,Camphor,Methyl salicylate and Eugenol in Fengyoujing.METHODS Agilent INNOWAX column and FID detector were used.The column temperature was programmed as maintaining 85℃ for 4 minutes and rising to 115℃ at a rate of 30℃·min-1and maintaining for 0 minutes,then rising to 200℃ at a rate of 70℃·min-1and maintaining for 4 minutes,rising to 250℃ at a rate of 100℃·min-1and maintaining for 3 minutes.RESULTS The linear ranges of Menthol,Cineol,Camphor,Methyl salicylate and Eugenol were within 0.34~17.22mg·mL-1（r=1.0000）,0.05~2.26mg·mL-1（r=1.0000）,0.04~1.93mg·mL-1（r=0.9999）,0.34~17.16mg·mL-1（r=1.0000）,0.04~1.80mg·mL-1（r=1.0000）.The average recoveries of were 96.17%（RSD0.65%）,96.69%（RSD1.23%）,101.14%（RSD1.65%）,96.86%（RSD0.68%）,98.02%（RSD0.88%）,respectively.Conlusion：The method is economy,efficient and accurate with a good reproduction,it can be used to determine the five compositions in Fengyoujing.