气相色谱法测定当归挥发油中藁本内酯的含量

目的建立当归挥发油中藁本内酯的含量测定方法。方法采用水蒸气蒸馏法进行挥发油的提取,以液体石蜡为吸收剂、无水乙醇为萃取剂,采用GC法对挥发油藁本内酯进行含量测定。气相色谱条件：AT-SE-54毛细管柱（15m×0.25mm×0.33μm）;氢焰离子化检测器（FID）;进样口温度230℃;检测器温度250℃;柱温以160℃为起始温度,保持2min,以10℃/min升温至200℃,保留2min。载气为氮气。进样量为1μL。结果在101.5μg·mL-1~609.0μg·mL-1范围内呈良好线性关系,峰面积与浓度的回归方程为：Y=522.23X＋5882.6（R2=0.999）,精密度试验RSD：1.76%,重复性试验RSD：0.70%,平均加样回收率96.49%。结论方法简便快速,试剂安全无毒,可用于当归挥发油中藁本内酯的含量测定。

Determination of Ligustilide in Volatile Fractions of Angelica Sinensis (Oliv.) Diels by Gas Chromatography

OBJECTIVE To establish a GC method for determination ligustilide in volatile fractions of Angelica sinensis（ Oliv.） Diels.METHODS The sential oil was extracted by steam distillation.Using liquid paraffin as ab-sorbent ,anhydrous ethanol as extractant.GC method was applied for the determination of ligustilide from the essential oil.Chromatographic conditionCapillary column AT-SE-54（15m ×0.25mm ×0.33μm） was used;The detectorwas FID;Inlet temperature was 230℃;The detector temperature was 250℃;Column temperature：160℃（2min） 10℃/min 200℃（2min）;Carrying gas was nitrogen;Injection volume was 1μL;RESULTS There is a good linear rela-tionshipin in 101.5μg· mL-1 ~609μg· mL-1 range,the peak area and the concentration of the regression equa-tion：Y=522.23X＋5882.6（R2 =0.9990）,precision test RSD was 1.76%,reproducible test RSD was 0.70%,the average recovery rate was 96.49%.CONCLUSION This method is simple ,quick and specific ,reagent is safe and non-toxic.It can be applied for determination ligustilide in volatile fractions of Angelica sinensis（ Oliv.） Diels.