血管平滑肌细胞在几种基质膜片上生长情况及三种接种培养方法的比较

目的 筛选适合于组织工程血管构建的可降解基质材料及最佳的接种培养方式。方法 将血管平滑肌细胞接种于几种现有的可降解基质材料上，采用MTT法观察细胞在几种基质材料上的生长情况；比较静态、水平摇床、旋转接种三种接种培养方式。结果 细胞相容性比较好的材料有：PGA、胶原加黏多糖、脱钙骨等。三种接种方法的接种率为：静态1．5％。水平摇床7．2％，旋转接种培养53．5％。结论 PGA、胶原加黏多糖、脱钙骨三种材料适合于组织工程血管再造的应用。而三种接种培养方式以旋转接种培养的效率最高。

The comparison of vascular smooth muscles cells in degradable materials and different seeding methods of the cells

Objective To select degradable materials and best seeding method for tissue engineering blood vessel. Methods Vascular smooth muscles cells were seeded onto these degradable scaffolds, MTT method was used to analyze the growth state of the co-cultured cells. Optimizing seeding methods were also be compared. Results SMCs grew better on PGA, collage combined with GAG and decalcifying bone, than the others. The efficiency of seeding is: static culture 1.5%; stired culture 7.2%; dynamic culture 53.5%. Conclusion PGA, collage combined with GAG, decalcifying bone are relatively compatible with the cells; Dynamic rotation seeding culture has highest seeding efficiency.