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目的 探讨牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)W83、ATCC33277刺激人牙周膜成纤维细胞(HPDLFs)分泌基质金属蛋白酶-1(MMP-1)、金属蛋白酶组织抑制剂-1(TIMP-1)的变化.方法 本研究于2010年9月至2011年6月在中国医科大学口腔医学院中心实验室进行.将P.gingivalis W83、ATCC33277作用于HPDLFs0、6、12、24、48h后,运用酶联免疫吸附法(ELISA)检测细胞上清液中MMP-1、TIMP-1质量浓度变化,并计算MMP-1/TIMP-1值.结果 P.gingivalis感染HPDLFs的MMP-1和TIMP-1表达均增强,并呈时间依赖性；且MMP-1/TIMP-1值明显高于未感染的对照组(P＜0.05).P.gingivalis W83感染后MMP-1/TIMP-1值明显高于P.gingivalis ATCC33277(P＜ 0.05).结论 P.gingivalis具有促进HPDLFs分泌MMP-1、TIMP-1的作用,且可造成牙周组织破坏；P.gingivalis W83降解细胞外基质的能力高于P.gingivalisATCC33277.

Effect of Porphyromonas gingivalis on the expression of MMP-1 and TIMP-1 in human periodontal ligament fibroblasts

Objective To investigate the effects of Porphyromonas gingivalis on the expression of MMP-1 and TIMP-1 in human periodontal ligament fibroblasts through using an in vitro model.Methods MMP-1 and TIMP-1 protein levels in culture supernatant were determined by ELISA at the different time intervals(6 h,12 h,24 h,48 h) following continuous co-culture of P.gingivalis W83 and ATCC33277 with HPDLFs.The ratio of MMP-1 /TIMP-1 was calculated.Results The expression of MMP-1 and TIMP-1 was enhanced in HPDLFs infected with P.gingivalis;the ratio of MMP-1/TIMP-1 in infected HPDLFs was significantly higher than that of control group(P < 0.05).The ratio of MMP-1/TIMP-1 in HPDLFs infected with P.gingivalis W83 was significantly higher than that in HPDLFs infected with P.gingivalis ATCC33277(P < 0.05).Conclusion P.gingivalis can accelerate secretion of MMP-1 and TIMP-1 in HPDLFs,which causes periodontal tissue destruction.P.gingivalis W83 has stronger pathogenicity for periodontal disease by producing extracellular matrix in local.