寒凝血瘀对结肠癌肺转移模型小鼠肺组织VEGF、 MMP-2表达的影响

目的:观察寒凝血瘀状态下Balb/c 小鼠C26 人工血行肺转移组织血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP-2)表达.方法:将小鼠随机分为空白对照组、寒凝血瘀组、单纯荷瘤组、复合造模组,分别进行造模,通过免疫组化、Western Blot方法检测肺转移灶中VEGF、MMP-2的表达.结果:复合造模组小鼠基质金属蛋白酶-2(MMP-2)表达高于单纯荷瘤组,差异有统计学意义(P<0.05),复合造模组小鼠血管内皮生长因子(VEGF)表达较单纯荷瘤组小鼠有增高趋势,差异无统计学意义(P>0.05).结论:寒凝血瘀状态可能通过促使结肠癌小鼠肿瘤组织VEGF和MMP-2表达的上调,促使小鼠C26结肠癌血行肺转移,促进疾病进展.

Effect of Blood Stasis Due to Cold on the Expression of Lung Tissue VEGF and MMP-2 in Mice withMetastatic Lung Lesions from Colon Cancer

Objective:To investigate the role of blood stasis caused by cold in the expression of vascular endothe lial growth factor(VEGF)and matrix metalloproteinase 2(MMP-2) in lung tissues of Balb/c mice with metastatic lung cancer.Methods: Twenty-four Balb/c mice were equally randomized into 4 groups: normal control group,blood stasis group,colon cancer group,and blood stasis-cancer group.Models were established in other 3 groups except the normal controls.The expression of VEGF was detected with immunohistochemistry and MMP-2 with Western blotting.Results: The expression of MMP-2 in the blood stasis-cancer group was significantly high er than that in the cancer group(P<0.05).The expression of VEGF in the blood stasis-cancer group was higher than that in the cancer group without a significant difference(P>0.05).Conclusion: The state of blood stasis caused by cold may prompt the hematogenous metastasis of colon cancer to the lung by upregulating the levels of VEGF and MMP-2 in tumor tissues.