蜂毒素对人胃癌细胞株BGC-823凋亡及其细胞周期的影响

目的：观察蜂毒素体外对人胃癌细胞株BGC-823增殖、凋亡及细胞周期的影响。方法：采用MTT法观察不同浓度的蜂毒素（32,16,8,4,2μg/mL）体外对人胃癌细胞株BGC-823增殖的影响,并用流式细胞仪检测其诱导的细胞凋亡和周期阻滞效应。结果：①与对照组比较,蜂毒素有抑制人胃癌细胞株BGC-823增殖的作用（P〈0.01）,随着药物浓度的增大,其对细胞的抑制率也明显增大,呈现剂量依赖性。②蜂毒素浓度从4μg/mL开始,即可诱导细胞产生凋亡,随着剂量的增加诱导凋亡的效果更加明显。③在细胞周期方面,与阴性对照比较,蜂毒素作用24小时后S期细胞比例上升,而G0/G1期比例明显下降,蜂毒素浓度达到16μg/mL时G0/G1期前出现明显的亚二倍体区。结论：蜂毒素对人胃癌细胞株BGC-823的增殖具有抑制作用,且能够引起该细胞的凋亡及改变其细胞周期。

Effects of Melittin on Apoptosis and Cell Cycle of Human Gastric Cancer Cell Line BGC-823

Objective： To investigate effect of melittin in vitro on proliferation,apoptosis and cell cycle of human gastric cancer cell line BGC-823.Method： Effects of different concentrations of melittin（32,16,8,4 or 2 μg/mL） on proliferation of human gastric cancer cell line BGC-823 were investigated with MTT assay;induced apoptosis and cycle blocking were detected with flow cytometer（FCM）.Result： ① Compared with control group, melittin inhibited proliferation of human gastric cancer cell line BGC-823（P〈0.01）.The inhibition effects increased with the dose.② Melittin induced cell apoptosis when the concentration reached to 4 μg/mL,the apoptosis-inducing effect rose with the dose of melittin.③ As cell cycle,the percentage of cells in S phase increased after melittin working for 24 hours while the ratio of the cells in G0/G1 phase decreased compared with negative control group, when the concentration of melittin reached to 16 μg/mL,the sub-diploid peak showed obviously before G 0 /G 1 phase.Conclusion： Melittin could inhibit the proliferation of human gastric cancer cell line BGC-823,induce the apoptosis of cells and change its cell cycle.