B16黑色素瘤皮内移植瘤模型的建立

背景与目的： 建立B16黑色素瘤细胞(简称B16细胞)皮内移植瘤模型。 材料与方法： 制备商品源B16细胞悬液，按每只C57BL鼠接种0.1 ml (3×105个)B16细胞于后肢外侧皮内；分离、培养移植瘤源B16细胞，倒置显微镜下观察细胞形态。 结果： 移植瘤出现时间为(9.6±1.2)d，致瘤率为100％；皮内注射B16细胞18 d内，肿瘤形态呈圆形或类圆形，边缘清楚；移植瘤源原代B16细胞多为圆形、类圆形或短梭形，商品源B16细胞为梭形或不规则形；HE染色表明移植瘤内有大量B16细胞。 结论： B16细胞皮内移植瘤模型易于对肿瘤生长的观察和测量，是早、中期肿瘤研究的合适模型之一。

Intradermal Model of B16 Melanoma in Mice

BACKGROUND AND AIM: To establish an intradermal model with transplanting melanoma B16 cells in C57BL mice． MATERIALS AND METHODS: commercially-acquired 3×106 B16 cells in 0.1 ml were inoculated intradermally in the right hindlimb in C57BL mice．B16 cells from tumors were isolated, cultured and examined. RESULTS: Tumor formation time was found to be (9.6±1.2)d and tumor formation rate was 100％. Within 18 d, tumors was round or round-like shape with distinct borders. Commercially-acquired B16 cells were mostly spindle or irregular whilst B16 cells from tumors were round or round-like or short-spindle. HE staining showed that there were a large number of B16 cells in the transplanted tumors. CONCLUSION: Intradermal tumor model of B16 cells in C57BL mice is helpful to monitor tumor growth in early and middle stages and is one of ideal models in tumor studies.