抗抑郁药物对慢性应激抑郁模型大鼠海马蛋白激酶C表达的干预作用

目的探讨氟西汀与噻奈普汀对慢性应激模型大鼠海马蛋白激酶C(PKC)表达的影响。方法将60只雄性Wistar大鼠随机分为抑郁模型组、氟西汀组、噻奈普汀组和正常对照组,各15只。模型组、氟西汀组和噻奈普汀组大鼠给予21 d的应激刺激,此期间正常对照组正常饲养,刺激期间氟西汀组每天灌胃氟西汀(10 mg/kg),噻奈普汀组每天灌胃噻奈普汀(50 mg/kg),模型组和正常对照组大鼠每天灌胃等体积的0.9%氯化钠注射液。行为学检测应用开场法和液体消耗实验。采用Western-blotting法检测各组大鼠海马PKC的表达情况。结果应激后,模型组大鼠水平穿越格数、竖立次数、修饰次数、糖水消耗百分比均显著低于正常对照组(P<0.01);氟西汀组水平穿越格数、竖立次数、修饰次数和糖水消耗百分比均显著高于模型组(P<0.05);噻奈普汀组糖水消耗百分比显著高于模型组(P<0.05)。在Western-blotting法检测中,模型组大鼠海马PKC的表达水平显著低于正常对照组(P<0.01);氟西汀组大鼠海马PKC的表达水平显著高于模型组(P<0.01),与正常对照组比较差异无统计学意义(P>0.05);噻奈普汀组大鼠海马PKC的表达水平显著高于模型组(P<0.01),但仍显著低于正常对照组(P<0.01)。结论氟西汀可以逆转慢性应激抑郁模型大鼠海马中PKC表达的降低;噻奈普汀可以部分逆转慢性应激抑郁模型大鼠海马中PKC表达的降低。

Effects of Fluoxetine and Tianeptine on the Expression of PKC in Hippocampus of Chronic Stress Depression Rats

Objective To investigate the effects of fluoxetine and tianeptine on the expression of PKC in hippocampus of chronic stress depression rats. Methods 60 male Wistar rats were randomly divided into depression group,fluoxetine group,tianeptine group and control group with each group 15 rats.The rats in depression group,fluoxetine group and tianeptine group were given stress stimulation for 21 days,and meanwhile rats in control group were kept normally.The rats in fluoxetine group were given fluoxetine by intragastric administration(10 mg/kg),rats in tianeptine group were given tianeptine by intragastric administration(50 mg/kg).While the depression group and control group were given same volume of 0.9% sodium chloride injection.The ethology examination was performed by using method of open-field and experiment of fluid consumption.The expression of PKC was detected by Western-blotting method. Results After the stress stimulation,horizontal crossing numbers,erection times,modification times and percentage of sacchar-consumption of the rats in depression group were significantly less than those of the control group(P<0.01).The horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of the rats in fluoxetine group were significantly less than those of the depression group(P<0.05).The percentage of sacchar-consumption of the rats in tianeptine group was significantly more than that of the depression group(P<0.05).In the test with Western-blotting method,the expression level of PKC in the hippocampus in depression group was significantly lower than that of the control group(P<0.01).The expression level of PKC in the hippocampus in fluoxetine group was significantly higher than that of the depression group(P<0.01),but no statistically significant difference showed between fluoxetine group and control group.The expression level of PKC in the hippocampus in tianeptine group was significantly higher than that of the depression group(P<0.01),but significantly lower than that of the control group(P<0.01). Conclusion Fluoxetine can reverse the decrease of the expression of PKC in hippocampus of chronic stress depression rats while tianeptine can also partly have the same function.