| 蛇毒精氨酸酯酶Agkihpin上调人肝癌细胞株SMMC-7721中表皮钙黏素的表达研究 |
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| 引用本文： | 黄程新,许淑茹,胡启平,马军,方玲,袁志刚,李晓龙.蛇毒精氨酸酯酶Agkihpin上调人肝癌细胞株SMMC-7721中表皮钙黏素的表达研究[J].中国全科医学,2012,15(9):1002-1005. |
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| 作者姓名： | 黄程新 许淑茹 胡启平 马军 方玲 袁志刚 李晓龙 |
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| 作者单位： | 1. 广西医科大学细胞生物学与遗传学教研室,广西南宁市,530021
2. 漳州卫生职业学院 |
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| 摘 要： | 目的探讨蛇毒精氨酸酯酶Agkihpin对人肝癌SMMC-7721细胞株中表皮钙黏素(E-CD)表达的影响及Agkihpin抑制人肝癌SMMC-7721细胞的机制。方法采用不同浓度的Agkihpin处理肝癌细胞株72 h后,应用免疫细胞化学、Western Blotting和逆转录PCR(RT-PCR)法检测E-CD在SMMC-7721细胞中的表达。结果不同浓度Agkihpin作用SMMC-7721细胞72 h后E-CD表达均上升,差异有统计学意义(P<0.05),并呈现出一定的剂量依赖效应。Agkihpin可显著上调SMMC-7721细胞中E-CD表达。结论在人低分化肝癌SMMC-7721细胞株中,Agkihpin能促进E-CD的表达,因而可能抑制肝癌细胞的迁移和降低肝癌组织的恶性程度。
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| 关 键 词： | 表皮钙黏素 精氨酸酯酶 肝肿瘤 蛇毒液类 |
Research on Agkihpin Up-Regulating the Expression of E-Cadherin in Hepatocellular Carcinoma SMMC-7721 Cell Strain |
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| Institution: | HUANG Cheng-xin,XU Shu-ru,HU Qi-ping,et al.Department of Cell Biology and Genetics,Guangxi Medical University,Nanning 530021,China |
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| Abstract: | Objective To explore the effects of Agkihpin on the expressions of E-Cadherin in hepatocellular carcinoma SMMC-7721 cell strain and to describe the mechanism of Agkihpin inhibiting SMMC-7721.Methods The hepatocellular carcinoma cell strain was treated with different concentrations of Agkihpin for 72 h.The expressions of E-Cadherin in SMMC-7721 cell strain were assayed by immunocytochemistry,Western Blotting and RT-PCR.Results E-Cadherin expressions in SMMC-7721 cells were all significantly increased after 72 h treatment by different concentrations of Agkihpin(P<0.05),and dose dependent effect was shown in the process.Agkihpin could significantly increase the expressions of E-CD in SMMC-7721 cells.Conclusion Agkihpin can promote the expressions of E-Cadherin in SMMC-7721 cells,which might be one of the reasons for Agkihpin inhibiting the cell migration of hepatocellular carcinoma cell and decreasing the grade malignancy of hepatocellular carcinoma. |
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| Keywords: | E-Cadherin Arginine esterase Liver neoplasms Snake venoms |
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