宁夏回族人群脂联素水平及基因单核苷酸多态性与冠心病相关性研究

目的探讨宁夏回族人群血浆脂联素(APN)水平及其单核苷酸基因多态性(SNP45T/G和SNP276G/T)两个位点与冠心病(CHD)之间的关系。方法将193例回族研究对象根据造影结果和临床资料分为2组:CHD组95例和正常对照组98例;应用酶联免疫吸附法及聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测回族正常对照组及CHD组血浆APN水平及APN2个位点基因多态性。结果回族CHD组APN水平降低;回族CHD组SNP+45TG+GG基因型和G等位基因的分布频率明显高于正常对照组,回族CHD组SNP+276TG+TT基因型和T等位基因的分布频率明显高于正常组,差异均有统计学意义(P<0.05)。回族SNP+45TG+GG基因型血浆APN水平低于TT基因型者,回族SNP+276TT+TG基因型APN水平低于GG基因型,差异均有统计学意义(P<0.05)。结论回族CHD组APN水平降低;回族CHD组SNP+45TG+GG基因型和G等位基因的分布频率明显高于正常对照组;回族CHD组SNP+276TG+TT基因型和T等位基因的分布频率高于正常组。

Correlation of Single Nucleotide Polymorphism of Adiponectin Gene with Coronary Artery Disease in Hui Ethnic Group in Ningxia

Objective To study Adiponectin(APN) plasma level and the correlation of its single nucleotide gene polymorphism(SNP45T/G and SNP276G/T) with coronary heart disease(CHD) in Hui ethnic group in Ningxia.Methods A total of 193 Hui patients were divided into two groups based on the coronary angiography and the clinical data:coronary heart disease(CHD) group(n=95) and normal control group(n=98).Enzyme-linked immunosorbent assay(ELISA) and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) were used to detect the plasma APN level and the gene polymorphism of its two sites.Results Compared with the normal control group,the plasma APN level in CHD group was reduced.The distribution frequencies of SNP+45 TG+GG genotype and G allele in CHD group were significantly higher,and the distribution Frequencies of SNP+276TG+TT genotype and T allele were also significantly higher(P<0.05).The plasma APN level of SNP+45TG+GG genotype was significantly lower than that of TT genotype and the plasma APN level of SNP+276TG+TT genotype was significantly lower than that of GG genotype(P<0.05).Conclusion The plasma APN level is low in Hui CHD patients.The distribution frequencies of SNP+45TG+GG genotype and G allele in Hui CHD patients may increase and the distribution frequencies of SNP+276TG+TT genotype and T allele may decrease.