地特胰岛素对乳腺癌MCF-7和MDA-MB-231细胞生长的影响研究

目的探讨地特胰岛素对人乳腺癌MCF-7和MDA-MB-231细胞生长的影响及涉及的信号通路。方法地特胰岛素不同浓度、时间作用MCF-7和MDA-MB-231细胞,MTT法检测细胞数目,Western blot法检测细胞外信号调节蛋白激酶1/2(Erk1/2)和蛋白激酶B(Akt)磷酸化程度,流式细胞仪检测细胞周期。结果与正常对照相比,地特胰岛素1 U/L的地特胰岛素作用24 h,抑制MCF-7和MDA-MB-231细胞增殖不明显,1、10、100、1 000 U/L的地特胰岛素作用48 h和1 U/L的地特胰岛素作用48、72、96 h,促进两种细胞增殖的作用不明显,差异均无统计学意义(P>0.05)。10 U/L地特胰岛素作用MCF-7和MDA-MB-231细胞24 h细胞周期变化,Erk1/2和Akt磷酸化程度较正常对照组差异均无统计学意义(P>0.05)。结论地特胰岛素对MCF-7和MDA-MB-231细胞增殖作用不明显,Erk1/2和Akt的磷酸化程度无明显升高。

Effects of Insulin Detemir on the Growth of Breast Cancer Cells MCF-7 and MDA-MB-231

Objective To investigate the effects of insulin Detemir on the growth of breast cancer cells MCF-7 and MDA-MB-231 and involved cell signaling pathways.Methods The breast cancer cells MCF-7 and MDA-MB-231 were treated by insulin Detemir with different concentration and action time.Cell proliferation was studied by MTT assay,phosphorylation of Akt and Erk1/2 were evaluated by western blot and cell cycle was measured by flow cytometry.Results Different concentration of insulin Determir showed no statistically significant difference on the growth of MCF-7 and MDA-MB-231 cells(P>0.05).Different action time of insulin Determir also showed no statistically significant difference on the growth of MCF-7 and MDA-MB-231 cells(P>0.05).Compared with the control group,the 24 h cell cycle,phosphorylation of Erk1/2 and Akt of MCF-7 and MDA-MB-231 cells showed no statistically significant difference(P>0.05).Conclusion Insulin Detemir cannot stimulate the proliferation of MCF-7 and MDA-MB-231 cells significantly.The phosphorylation of Akt and Erk1/2 are not significantly increased.