KZY-2复方制剂对白念珠菌芽管形成的影响

目的：探讨抗真菌中药复方制剂（KZY-2）对白念珠菌芽管形成的抑制作用。方法：采用白念珠菌芽管形成的优化条件（1640培养基、pH7．0、37℃振荡培养3h），观察药物对白念珠菌标准菌株（ATCC14053、CCCMC1a）和临床分离菌株（C1-1、C1-4）芽管形成的抑制程度。结果：（1）KZY-2对四株实验菌均具有明显抑制芽管形成的作用，但对不同菌株芽管形成的最低抑菌浓度（MIC）差异较大，对ATCC14053、CCCMC1a、C1-1、C1-4的MIC依次为：31．25μg／ml；62．5μg／ml；125μg／ml；500μg／ml。组间比较具有显著意义（P〈0．05或P〈0．01）。（2）药物浓度可明显影响抑制芽管形成的百分率，浓度在50％MIC以下时，芽管形成抑制率均低于30％。结论：KZY-2复方制剂具有明显抑制白念珠菌芽管形成的作用，抑制作用的强弱与药物浓度密切相关。

Effects of KZY-2 compound on bud formation in Candida albicans

Objective： To investigate the inhibition of bud formation in Candida albicans about the anti -fungal drug （KZY-2 compound）. Methods： （ 1 ） By means of the optimum conditions which were 1640 medium, pHT. 0 and 37℃ oscillated culture for 3 hours of bud formation in Candida albicans, to observe the effects of the drug on the standard strains （ATCC14053, CCCMC1 a） and the clinical isolation strains （C1-1, C1-4）of candida albicans. Results： （1） Bud formation in all the strains was significantly inhibited, while the minimum inhibitory concentrations （MIC） of ATCC14053, CCCMC1a,C1-1 and C1-4 which were significantly different were respectively 31.25 μg/ml,62.5 μg/ml, 125 μg/ml and 500μg/ml. （2）The percentage of bud formation was significantly affected by the drug concentration ,which was less than 50% MIC, the inhibited rates of bud formation were all below 30 percent. Conclusions： KZY- 2 compound could significantly inhibit bud formation in Candida albicans and the degree of inhibition was closely related with the drug concentration.