白英水提物诱导人宫颈癌HeLa细胞凋亡的实验研究

目的:观察白英水提物诱导人宫颈癌HeLa细胞凋亡的作用,并初步探讨其分子机制。方法:常规方法制备白英水提物,体外培养人宫颈癌HeLa细胞。实验组设白英水提物12.5、25.0、50.0mg/mL3种浓度,以顺铂(25.0mg/L)为阳性对照组。采用细胞毒试验(MTT法)检测HeLa细胞抑制率,荧光显微镜观察HeLa细胞形态变化,琼脂糖凝胶电泳检测DNA梯形泳带,半定量RT-PCR法检测凋亡相关基因survivin mRNA和caspase-3mRNA表达的变化。结果:白英水提物对HeLa细胞增殖有抑制作用,且呈明显的时效和量效关系,并诱导其发生凋亡,表现为细胞核固缩、染色质凝集、边聚等。琼脂糖凝胶电泳显示DNA梯形泳带,半定量RT-PCR法检测显示survivin mRNA表达下调、caspase-3mRNA表达上调。结论:白英水提物对HeLa细胞有较强的增殖抑制和诱导凋亡作用,其诱导HeLa细胞凋亡的分子机制可能与上调caspase-3基因表达、下调survivin基因表达有关。

Apoptosis-induced effect of solanum lyratum thunb extract on human cervical cancer hela cells

AIM:To investigate the apoptosis-inducing and proliferation inhibition effect of solanum lyratum thunb(SLT) extract and its molecular mechanism on human cervical cancer HeLa cells.METHODS:Dried whole herbs of SLT were extracted by boiling distilled water.Human cervical cancer HeLa cells were randomly divided into the control group,SLT-treated groups(12.5,25.0,50.0 mg/mL) and DDP(25.0 mg/L) group.Morphological changes of apoptosis were observed by fluorescence microscope.DNA ladders were examined by DNA agarose gel electrophoresis and the expression of survivin mRNA and caspase-3 mRNA were detected by semi-quantitative RT-PCR.RESULTS:SLT extract displayed a strong proliferation inhibitory effect in a dose-and-time-dependent manner in HeLa cells.Nuclear shrinkage,chromatin condensation and margination were observed under a fluorescence microscope in HeLa cells treated with SLT extract and DDP.Survivin mRNA levels were down-regulated while caspase-3 were up-regulated.CONCLUSION:SLT extract displays a strong proliferation inhibitory and apoptosis-inducing effect in a dose-and-time-dependent manner in HeLa cells.The molecular mechanism of apoptosis of HeLa cells induced by SLT extract has possibly related to up-regulating expression of caspase-3 gene and down-regulating survivin gene.