妊娠高血压综合征患者胎盘肝细胞生长因子的表达与胎盘血管发生

目的　探讨肝细胞生长因子 (hepatocytegrowthfactor,HGF)在正常妊娠妇女胎盘和妊高征患者胎盘组织中的表达及对妊高征胎盘绒毛血管发生的影响。　方法　不同程度妊高征晚孕和正常妊娠晚孕孕妇共 78例 ,采用免疫组织化学SP法检测胎盘组织HGF的表达强度 ,同时作HE染色 ,选取5个高倍视野 ,计数绒毛血管数。　结果　HGF主要表达于绒毛间质细胞胞浆 ,蜕膜细胞有少量表达。重度妊高征患者胎盘绒毛间质细胞HGF表达强度 ( )为 1例 ,显著低于正常晚孕组的表达强度( )为 4例 (H =7.395 ,P =0 0 0 3) ,而轻度及中度妊高征组HGF表达强度 ( )均为 2例与正常晚孕组比较 ,差异无显著性 (H =0 .86 9,P =0 .35 1,H =0 .0 17,P =0 .896 )。不同程度妊高征胎盘绒毛血管密度均较正常孕妇减少 ,重度妊高征组绒毛血管密度为 (5 7.5 1± 15 .4 9)个 / 4 0 0×与正常晚孕组(6 7.91± 16 .90 )个 / 4 0 0×相比 ,差异有显著性 (F =10 .6 76 ,P =0 .0 0 8)。相关分析显示 ,胎盘间质细胞HGF表达强度与绒毛血管密度呈正相关 (r =2 2 4 6 ,P =0 0 30 )。　结论　HGF在胎盘中主要由绒毛间质细胞分泌 ,妊高征患者HGF分泌减少可能是引起胎盘血管网减少的原因之一。

Expression of hepatocyte growth factor in placenta in PIH

Objective To study the expression of hepatocyte growth factor (HGF) in placenta from normal pregnancy and pregnancy induced hypertension (PIH) and the effect of HGF on angiogenesis in placenta. Methods Immunohistochemistry was used to determine the location and intensity of HGF staining in placenta from 20 cases of normal pregnancy and 58 cases of mild,moderate and severe PIH groups. HE staining was used in order to count the number of the vessles. Results HGF mostly were expressed in villous core mesenchymal cells,less in decidual cells . Intensity of HGF immnostaining in villous mesenchymal cells was significantly reduced in the severe PIH group compared with the normal group(H=7.395,P=0.003),while there were no statistical differences between the mild,moderate PIH group and the normal group (H=0.869,P=0.351,H=0.017,P=0.896) .The count of vessels reduced in severe PIH (F=10.676,P=0.008). There was positive correlation between the expression of HGF and the count of vessels in villous(r=0.246,P=0 030). Conclusion These results suggested that HGF is secreted mainly by villous core mesenchymal cells in human placenta and the reduction of HGF in PIH may be responsible for the impaired vascularization in PIH.