氟离子注入钛表面改性对成骨细胞黏着斑形成的影响

目的探讨氟离子注入纯钛进行表面改性后，钛表面改性层化学组成的变化及其对人成骨细胞样细胞系MG- 63黏着斑形成的影响。方法应用等离子体浸没离子注入装置对钛试件进行氟离子注入，通过X线光电子能谱（XPS）分析研究其表面化学组成和各元素的化学结合价态。将人MG- 63细胞接种于氟离子注入组和纯钛组钛试件表面，用激光共聚焦显微镜观察两组钛试件表面对人MG- 63细胞黏着斑形成的影响。结果XPS全谱图显示氟离子注入后试件由钛、氧、氟和碳元素组成，拟合结果显示钛表面改性层的元素以二氧化钛和三氟化钛的形式存在。MG-63细胞培养6 h后，氟离子注入组钛试件表面的黏着斑形成数量比纯钛组钛试件表面形成得多，二者间差异具有统计学意义（P<0.05）。结论氟离子注入可成功地将氟元素引入纯钛表面形成含有二氧化钛和三氟化钛的表面改性层，该层可促进成骨细胞黏附初期黏着斑的形成，提示其具有良好的生物相容性。

Influence of fluoride ion-implanted titanium on the formation of focal adhesion plaque in vitro

Objective To evaluate the chemical composition of the modified surface of fluoride ion-implanted titanium and assess the effect on the formation of focal adhesion plaque in vitro.Methods Pure commercial titanium discs were treated with fluoride ion implantation by plasma immersion ion implantation technique(PIII) and chemical composition and value of the surface modification layer were characterized by X-ray photoelectron spectrometer(XPS).In order to investigate the formation of focal adhesion plaque,MG-63 cells were seeded onto the surfaces of the modified Ti discs and quantified by morphometric analysis using an immunofluorescence microscope.Results The full range XPS spectra and fitting results indicated that the surface of fluoride ion-implanted titanium was the mixture of titanium dioxide and titanium trifluoride.Meanwhile,the quantity of focal adhesion plaque on fluoride ion-implanted titanium was more than that on the non-implanted titanium after 6 hours' cell culture.Conclusion The XPS data revealed that the modified surface layer of fluoride ion-implanted titanium contained titanium dioxide and titanium trifluoride,which could enhance the formation of focal adhesion plaque.