槐定碱诱导人胃癌MGC-803细胞凋亡的实验研究

目的 探讨槐定碱对人胃癌MGC-803细胞抑制作用及其诱导凋亡机理。方法 用MTT法测定槐定碱对MGC-803细胞的IC50，荧光显微镜、电子显微镜、流式细胞仪和DNA凝胶电泳等技术观察细胞凋亡。结果 实验显示0．4～3.2mg／ml的槐定碱对体外培养的MGC-803细胞有明显的抑制作用并可诱导其发生凋亡，凋亡细胞表现为细胞固缩，核染色质聚集或碎裂、胞质空泡化等；DNA电泳可见DNA“梯形”条带；流式细胞仪检测Sub-G1凋亡峰在G1期前出现，S期细胞比例增高。结论 槐定碱对体外培养MGC-803细胞生长有抑制作用，机理与通过阻止细胞周期的进程，诱导肿瘤细胞凋亡有关。

Apoptosis of gastric carcinoma MGC-803 cells induced by sophoridine

ObjectiveTo investigate the growth-inhibition and the mechanism of apoptosis induced by sophoridine on MGC-803 cell line. MethodsMTT assay was used to detect the half-inhibition concentration (IC_ 50 ), Fluorescence microscopy, electron microscopy, flow cytometry (FCM) and DNA gel electrophoresis were used to demonstrate the presence of apoptosis. ResultsThe experiment showed that 0.4-3.2mg /ml sophoridine could inhibit the growth of MGC-803 cells significantly, and morphological characteristics of apoptosis was observed with condensation of nucleus, bubble of cytoplasm, fragment of nucleus, and a ladder-like pattern of DNA fragmentation in agarose gel electrophoresis. Sub-G_1 peak and increased S period cells were shown by FCM. ConclusionSophoridine can induce the inhibition of cell growth by means of apoptosis, and the blocking of S period cells is involved in the mechanism of apoptosis.