肾虚雄性大鼠红细胞膜组分及跨膜信息传递变化的实验研究

目的探讨肾虚对细胞膜的结构和功能及其跨膜信息传递的影响以及补肾中药的作用机制。方法通过手术去势切除性腺造成雄性大鼠肾虚动物模型，施加补肾中药，以红细胞膜蛋白激酶C(PKC)、Ca

Experimental Study onConstitution of Erythrocyte Membrane and Change of Transmembrane Information Transmissionin Male Rats of Kidney asthenia

The constitution and function of erythrocyte membrane and the changes of transmembrane information transmission in rats of kidney asthenia were investigated.In this study,a model of male rat of kidney asthenia was established by excision of sexual gland.The activities of protein kinase C (PKC),Ca2+-Mg2+-ATPase,Mg2+-ATPase in erythrocyte membrane and content of membrane phospholipid were observed.The results show that the activities of protein kinase C(PKC)and Ca2+-Mg2+-ATPase in erythrocyte membrane in the model group are significantly lower than those in the control group(P＜0.01).The contents of PC and PI in erythrocyte membrane are significantly lower(P＜0.01),that of PS is significantly lower (P＜0.05) and that of SM is significantly higher(P＜0.01).The kidney-tonifying traditional Chinese drugs can increase the activities of protein kinase C(PKC) and Ca2+-Mg2+-ATPase,decrease the content of SM(P＜0.01) and increase the content of PC,PI,PS(P＜0.01).It is concluded that the change of constitution and function of membrane in male rats of kidney asthenia can have effect on the asymmetry,fluidity and transmembrane information transmission.The kidney-tonifying traditional Chinese drugs can increase the activities of some of enzyme in membrane,correct some of constitution of membrane,such as SM,PC,PI,which can restore the constitution and function of membrane and the transmembrane information transmission,thus have the preventive and therapeutic effect on the diseases of kidney asthenia.