| 放射治疗对小鼠肾癌细胞凋亡和Bcl-2与Bax蛋白表达的影响 |
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| 引用本文： | 王瑶,黄建华,吕海燕,郭佳,杨军,马林. 放射治疗对小鼠肾癌细胞凋亡和Bcl-2与Bax蛋白表达的影响[J]. 感染、炎症、修复, 2007, 8(1): 11-13,F0003 |
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| 作者姓名： | 王瑶 黄建华 吕海燕 郭佳 杨军 马林 |
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| 作者单位： | 解放军总医院基础医学研究所免疫学研究室,北京,100853;解放军总医院基础医学研究所放射治疗科,北京,100853 |
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| 摘 要： | 目的:观察放射治疗对BALB/c小鼠肾癌的抑制作用及对Bcl-2和Bax蛋白表达的影响.方法:建立BALB/c小鼠的肾癌细胞移植瘤放射治疗模型;HE染色测定肿瘤细胞死亡面积,采用免疫印迹法(western blot法)检测肿瘤组织的凋亡相关蛋白表达.结果:放疗组小鼠移植瘤生长速度较肿瘤对照组明显减慢.放疗组小鼠肿瘤组织中抗凋亡蛋白Bcl-2表达下调,促凋亡蛋白Bax表达上调.结论:经放射治疗后,小鼠肿瘤生长受到明显抑制,可能与放疗引发肿瘤细胞凋亡相关蛋白表达变化相关.
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| 关 键 词： | 放射治疗 Bcl-2 Bax 肾癌 |
| 修稿时间： | 2006-08-18 |
The expressions of apoptosis related genes Bcl-2, Bax protein and apoptosis of renal carcinoma cells after radiotherapy in mice |
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| Wang Yao,Huang Jianhua,Lv Haiyan,et al.. The expressions of apoptosis related genes Bcl-2, Bax protein and apoptosis of renal carcinoma cells after radiotherapy in mice[J]. Infection Inflammation Repair, 2007, 8(1): 11-13,F0003 |
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| Authors: | Wang Yao Huang Jianhua Lv Haiyan et al. |
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| Affiliation: | Wang Yao*,Huang Jianhua,Lv Haiyan,et al.*Department of Immunology,Institute Basic Medical Sciences,General Hospital of PLA,Beijing 100853,China |
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| Abstract: | Objective: To observe the inhibitory effect of radiotherapy on expressions of Bcl-2 and Bax protein of renal carcinoma in BALB/c mice. Methods: Renal carcinoma cells were implanted in BALB/c mice,and tumor volume and weight were measured . The expression of Bcl-2,Bax,and Bak protein in implanted renal tumors were assayed by Western blot. Results: Growth of tumor mass was markedly slower after radiotherapy than that in untreated mice. The expression level of Bax was remarkably increased,the expression of Bcl-2 pretein was significantly decreased after radiation. Conclusion: The growth of renal carcinoma is markedly retarded in mice after radiation,and it might be attributed to changes in apoptosis related genes. |
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| Keywords: | Radiotherapy Bcl-2 Bax Renal carcinoma |
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