失血性休克和再灌注大鼠多脏器细胞损伤的实验研究

为探讨再灌注损伤机制在失血性休克中的意义，方法：作采用失血性休克大鼠实验模型，测定和比较了大鼠不同脏器中丙二醛（ＭＤＡ），超氧化物歧化酶（ＳＯＤ）含量和血浆溶酶体水解酶活性在失血休克３ｈ，失血１．５ｈ后再灌注１．５ｈ的改变以及外源性ＳＯＤ和过氧化氢酶（Ｃａｔ）的防治再灌注损伤的效果，结果：失血性休克后快速再灌注，各脏器组织中ＭＤＡ和血浆溶酶体明显增高（Ｐ〈０．０１），ＳＯＤ显下降（Ｐ〈０．０１

Study on the cell injury of multiple organs in hemorrhagic shock andreperfused rats

Objective: In order to investigate the significance of mechanism of the reperfusion injury in hemor-rhagic shoc.Methods: The changes of MDA and superoxide dismutase (SOD)in various tissues and activityof lysosomal hydrolases in plasma of rat in sustained hemorrhagic shock for 3 h and reperfused for 1.5 h afterhemorrhage 1.5 h were measured and compared, the effect of pretreatment with exogenous SOD and catalase(Cat) was also evaluated. Results: In reperfused rats, the cellular damage was much more severe than thatoccured in sustained hemorrhage rats,the levels of MDA and lysosomal hydrolases increased significantly(P<0. 01) and levels of SOD obviously decreased (P<0.01). Pretreatment with combination of SOD and Catwas very effective in attenuating the reperfused injury.Conclusion : The mechanism of ischemia and reperfu-sion injury resulting from oxygen free radicals may be a primary factor of irreversible shock, which has an im-portant clinical significance for the shock rescue.