| 人胰岛素样生长因子Ⅱ基因P1、P3启动子克隆及意义 |
| |
| 引用本文： | 汤绍辉,杨冬华,舒建昌,黄卫.人胰岛素样生长因子Ⅱ基因P1、P3启动子克隆及意义[J].中国病理生理杂志,2003,19(12):1662-1666. |
| |
| 作者姓名： | 汤绍辉 杨冬华 舒建昌 黄卫 |
| |
| 作者单位： | 暨南大学附属第一医院消化科, 广东 广州 510630 |
| |
| 基金项目： | 国家自然科学基金资助项目 (No .30 0 70 85 3)，广东省自然科学基金资助项目(No.990 4 2 2 ) |
| |
| 摘 要： | 目的:人胰岛素样生长因子Ⅱ(IGF-Ⅱ)基因P1、P3启动子的克隆。方法:根据GenBank数据库提供的IGF-Ⅱ基因DNA全序列及有关文献, 应用巢式PCR技术从L-02正常人胎肝细胞系中扩增分离出P1、P3启动子片段, 并采用TOPO TA Cloning kit将PCR产物克隆入pCR2.1-TOPO T载体。结果:经琼脂糖凝胶电泳及直接测序鉴定, 克隆的IGF-Ⅱ基因P1、P3启动子片段碱基序列与GenBank数据库一致。结论:成功克隆了IGF-II基因P1、P3启动子。
|
| 关 键 词： | 胰岛素样生长因子Ⅱ 基因 启动子区(遗传学) 克隆 分子 |
| 文章编号： | 1000-4718(2003)12-1662-05 |
| 收稿时间： | 2003-04-23 |
Cloning of P1 and P3 promoters of human insulin-like growth factor Ⅱ gene |
| |
| TANG Shao-hui,YANG Dong-hua,SHU Jian-chang,HUANG Wei.Cloning of P1 and P3 promoters of human insulin-like growth factor Ⅱ gene[J].Chinese Journal of Pathophysiology,2003,19(12):1662-1666. |
| |
| Authors: | TANG Shao-hui YANG Dong-hua SHU Jian-chang HUANG Wei |
| |
| Institution: | Department of Gastroenterology, First Affiliated Hospital, Jinan University, Guangzhou 510630, China |
| |
| Abstract: | AIM:To clone P1 and P3 promoters of the human insulin-like growth factor Ⅱ(IGF-Ⅱ) gene. METHODS:According to the complete DNA sequence of IGF-Ⅱ gene, the nested primer PCR was performed for amplifying P1 and P3 promoter fragments of the gene from human L-02 cell line.These PCR products were analyzed by agarose gel electrophoresis, and cloned by using TOPO TA Cloning kit. The positive clones containing P1 and P3 fragments were selected and confirmed by sequencing.RESULTS:The DNA sequences of P1 and P3 promoters cloned were accordant with GenBank data. CONCLUSION:In this study P1 and P3 promoters of the IGF-II gene were cloned successfully. |
| |
| Keywords: | Insulin-like growth factor Ⅱ Genes Promoter regions (genetics) Cloning molecular |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国病理生理杂志》浏览原始摘要信息 |
| 点击此处可从《中国病理生理杂志》下载免费的PDF全文 |
