| 丙型肝炎病毒核心抗原检测试剂的研制及初步应用 |
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| 引用本文： | 王国华,张贺秋,李少波,刘劭钢,陈坤,宋晓国,凌世淦.丙型肝炎病毒核心抗原检测试剂的研制及初步应用[J].中国输血杂志,2004,17(5):299-301. |
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| 作者姓名： | 王国华 张贺秋 李少波 刘劭钢 陈坤 宋晓国 凌世淦 |
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| 作者单位： | 1. 军事医学科学院基础医学研究所,北京,100850
2. 湖南景达基因有限公司 |
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| 基金项目： | 十五国家科技攻关计划项目专题(2001BA705B060) |
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| 摘 要： | 目的 研制丙型肝炎病毒核心抗原 (HCV cAg)ELISA检测试剂 ,用于诊断早期丙型肝炎。方法 用基因工程表达的HCV cAg ,免疫Balb/c小鼠 ,制备抗HCV cAg单克隆抗体 ,建立检测HCV cAg双抗体夹心酶联免疫吸附试验 (ELISA) ;以此检测 12 5份抗 HCV、抗 HIV、抗 TP阴性 ,但单项ALT高的献血者血浆。结果 12 5份单项ALT高的献血者血浆中检出 9份HCV cAg阳性。结论 HCV cAg双抗体夹心ELISA试剂 ,有望用于早期丙型肝炎诊断。
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| 关 键 词： | 丙型肝炎病毒 核心抗原 丙型肝炎 酶联免疫吸附试验 单克隆抗体 |
| 文章编号： | 1004-549X(2004)05-0299-03 |
| 修稿时间： | 2003年12月4日 |
Preparation and testing of hepatitis C core antigen ELISA reagent |
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| WANG Guohua ZHANG Heqiu,LI Shaobo,et al..Preparation and testing of hepatitis C core antigen ELISA reagent[J].Chinese Journal of Blood Transfusion,2004,17(5):299-301. |
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| Authors: | WANG Guohua ZHANG Heqiu LI Shaobo |
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| Institution: | WANG Guohua ZHANG Heqiu,LI Shaobo,et al.Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Bejing 100850,China |
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| Abstract: | Objective To establish a sandwich enzyme-linked immunosorbent (ELISA) method for early diagnosis of hepatitis C. Methods Immunization of Balb/c mice with hepatitis C core antigen were prepared by genetic engineering. Mouse monoclonal antibodies (McAb) to anti-HCV-core Ag were obtained. A sandwich ELISA kit for detecting HCV-core Ag was developed by using four strains of anti-HCV-core Ag McAb. One hundred and twenty five serum specimens with increased ALT but negative for anti-HCV, anti-HIV, and anti-Tp tests were tested. Results Nine of the 125 specimens were positive for HCV-core Ag. Conclusion The double sandwich ELISA kits for detecting HCV-core Ag may be useful for the early diagnosis of hepatitis C . |
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| Keywords: | Hepatitis C virus Hepatitis C core antigen Monoclonal antibody |
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