EIA定量检测T_3、T_4的方法学评价和临床应用

目的 :了解酶免疫法定量检测血清中三碘甲状腺原氨酸 (T3 )和甲状腺素 (T4 )的可行性。方法 :采用酶免疫法和放射免疫法检测 31份血清的T3 和T4 含量 ,其结果与放射免疫法的检测结果作对照 ,同时对一定数量的血清标本作重复试验、回收试验和干扰试验。结果 :酶免疫法灵敏度T3 :0 3nmoL/L ,T4 :5 2nmoL/L ;血清标本批内CV值和批间CV值 :T3 为7 5 %和 9 3% ,T4 为 5 6 %和 8 4% ;T3 平均回收率为 10 3% ;T4 平均回收率 10 4%。应用EIA及RIA共测定临床标本 31例 ,同时进行相关性检验 ,两法结果无显著性差异 (P >0 0 5 )。结论 :酶免疫法用于血清T3 和T4 定量测定具有灵敏度高和准确性强 ,实验干扰因素较易控制等特点。

The Evaluation and Clinical Application of EIA in the Quantitative Determination of T_3 and T_4

Objective: To investigate the possibility of enzyme immunoassay(EIA)in the quantitative determination of serum triiodothyronine(T 3)and thyroxine(T 4).Methods:Serum samples of 31 cases were detected by EIA,the results were compared with that detected by radioimmunoassay(RIA).Repeated test,recovered test and interfered test were engaged in other samples.Results:The sensitivity of EIA was 0 3nmoL/L in T 3 and 5 2nmoL/L in T 4.The coefficients of variation(CV)for T 3 were 7 5% and 9 3% in one batch inside and in different batches separately, which of T 4 were 5 6% and 8 4%,separately.The average rates of recovery were 103% in T 3 and 104% in T 4.There was no significant difference between EIA and RIA( P >0 05).Conclusion:EIA can be used to detect serum T 3 and T 4 with sensibility and accuracy.