| 薄层色谱法测定夏枯草中熊果酸的含量 |
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| 引用本文： | 李培毅 牛艳艳 贾力莉 冯玛莉 武玉鹏. 薄层色谱法测定夏枯草中熊果酸的含量[J]. 山西中医学院学报, 2000, 1(2): 54-55 |
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| 作者姓名： | 李培毅 牛艳艳 贾力莉 冯玛莉 武玉鹏 |
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| 作者单位： | 山西省中医药研究院中心实验室!山西太原030012 |
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| 摘 要： | 目的 :测定夏枯草中熊果酸的含量。方法 :采用双波长薄层扫描法。以环己烷∶氯仿∶醋酸乙酯∶冰醋酸 ( 2 0∶5∶8∶0 .5)为展开剂 ,选择λS=530nm ,λR=70 0nm作为熊果酸含量测定的扫描波长 ,对夏枯草中熊果酸的含量进行了测定 ,并对方法学进行了考察。结果 :加样回收测定熊果酸的平均回收率为 10 0 .32 % (RDS =2 .14 % )。结论 :此法重现性、回收率良好 ,专属性较强 ,是中药制剂中夏枯草质量控制较为理想的指标。
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| 关 键 词： | 夏枯草 薄层色谱 熊果酸 含量测定 |
Determine the content of ursolic acid in the spica prunellae by TLC scanning |
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| Li Peiyi et al.. Determine the content of ursolic acid in the spica prunellae by TLC scanning[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2000, 1(2): 54-55 |
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| Authors: | Li Peiyi et al. |
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| Abstract: | Objective: To establish a determination method for ursolic acid in spica prunellae. Methods:TLC scanning was used to determine the content of ursolic acid, using the cyclohexane: trichloromethane: ethyl acetate: glacial acetic acid(20∶5∶8∶0.5) as the developing system, 10% sulfuric acid-ethanol solution as the spray reagent, the detected by dual wavelength scanning (λ S=530nm, λ R=700nm). Results: The linearity of this method was well with the recovery of the added sample 100.32%(RSD=2.14%). Conclusion: This method is simple, quick, accurate and with good reproducibility, can be used for the quality control of spica prunellae in traditional Chinese patent Medicines. |
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| Keywords: | spica prunellae ursolic acid TLC scanning determine |
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