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| 可降解组织工程血管支架材料的细胞相容性 | |
| 引用本文： | 孙平平,张振亮,郭光伟,周建业.可降解组织工程血管支架材料的细胞相容性[J].中国现代医生,2010,48(22):83-85,F0003. |
| 作者姓名： | 孙平平 张振亮 郭光伟 周建业 |
| 作者单位： | 1. 山西医科大学第二临床医学院心胸外科,山西太原,030001 2. 山东潍坊医学院,山东潍坊,261053 3. 中国医学科学院阜外心血管病医院卫生部心血管再生医学重点实验室,北京,100037 |
| 摘 要： | 目的对可降解材料聚乳酸-羟基乙酸共聚物和聚氨酯体外评价,初步探讨其作为血管支架材料的可行性。方法通过血红蛋白的释放对溶血进行评价;采用细胞增殖度法(MTT法)、细胞形态学观察方法研究聚乳酸-羟基乙酸共聚物和聚氨酯材料的细胞毒性。结果 PLGA和PU组的溶血率均小于ISO规定的5%,可认为这两种材料无溶血作用。MTT比色法结果显示细胞毒性为0～1级;细胞形态学观察显示L929细胞在聚氨酯材料浸提液中呈梭形或三角形,贴壁良好。结论这两种材料溶血低,细胞相容性良好,符合组织工程血管支架材料的应用要求。 |
| 关 键 词： | 聚乳酸-羟基乙酸共聚物 聚氨酯 细胞毒性 溶血率 |
Cell Compatibility of Biodegradable Tissue Engineered Vascular Scaffold |
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| Authors: | SUN Pingping ZHANG Zhenliang GUO Guangwei ZHOU Jianye |
| Institution: | 1.Cardiothoracic Surgery,the Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China;2.Shandong Weifang Medical College,Weifang 261053,China;3.Research Center for Cardiac Regenerative Medicine,the Ministry of Health,Cardiovascular Institute and Fuwai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100037,China |
| Abstract: | Objective To evaluate the feasibility of the biodegradable polylacticglycolic-acid（PLGA） and polyurethane（PU） as tissue engineered vascular scaffold in vitro.Methods Hemolysis activity was assessed by determination of hemoglobin release.Cell compatibility was evaluated by MTT measurement and morphological observation.Results The haemolytic rates of both PLGA and PU groups were less than 5% of ISO requirements,suggesting these materials had no haematolysis effect.MTT colorimetry showed that the cytotoxic grade of the novel polyurethane was 0～1.The cell morphology observation showed that the L929 cells were spindle-shaped or triangular with good stretch.Conclusion The two sorts of materials with low hemolysis and good cell compatibility meet the application requirements for tissue engineered vascular scaffold. |
| Keywords: | Polylacticglycolic-acid Polyurethane Cytocompatibility Haemolytic rate |
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