乙二胺四乙酸二钠对晶状体上皮细胞清除作用的实验研究

目的:探讨乙二胺四乙酸二钠(EDTA)对晶状体囊膜上皮细胞的清除效果。方法:收集白内障超声乳化囊外摘除术中环形撕取的晶状体前囊膜32例,将其随机分为4组,分别用5mmol/L,10mmol/L,20mmol/L EDTA及生理盐水进行前囊膜消化处理,观察晶状体上皮细胞的清除情况。结果:经过20mmol/L EDTA溶液处理的晶状体前囊膜,上皮细胞可以完全脱落,经过5mmol/L,10mmol/L EDTA溶液处理的晶状体前囊膜,上皮细胞部分脱落,生理盐水对照组仅见少量晶状体上皮细胞脱落。结论:EDTA可以清除晶状体囊膜的上皮细胞,并随浓度增加效果增强,该结果可为临床应用EDTA清除晶状体上皮细胞以防治后发性白内障(posterior capsular opacification,PCO)的发生提供实验基础和理论依据。

Study of the effection of EDTA on lens epithelial cells removal

AIM:To study the effection of EDTA on lens epithelial cells removal.METHODS:Thirty-two anterior lens capsules were collected in phacoemulsification,then randomly divided into 4 groups.Anterior lens capsules were digested with 5mmol/L,10mmol/L,20mmol/L EDTA and saline.Clearance situation of lens epithelial cells were observed.RESULTS:Epithelial cells could be completely detached from the anterior lens capsule by 20mmol/L EDTA solution treatment,some epithelial cells shed by the 5mmol/L and 10mmol/L EDTA solution treatment.In the control group only a small amount of epithelial cells shed.CONCLUSION:EDTA can remove the epithelial cells of anterior lens capsule.It can afford experimental and theoretical basis for clinical application of EDTA to remove the lens epithelial cells and prevent posterior capsular opacification(PCO).