反相高效液相色谱法测定桔梗中3种三萜皂苷类化合物的含量

目的建立RP-HPLC法同时测定桔梗中去芹糖桔梗皂苷E、桔梗皂苷E和桔梗皂苷D3的含量。方法以Hypersil-ODS(4.6 mm×250 mm,5μm)为色谱柱,流动相为乙腈(A)-水(B),梯度洗脱,流速为0.8 mL.min-1,检测波长210 nm,温度为室温。结果去芹糖桔梗皂苷E、桔梗皂苷E和桔梗皂苷D3分别在4.160~83.2、10.75~215.0、4.080~81.6μg.mL-1与峰面积线性关系良好,平均回收率分别为96.5%、97.0%和97.3%,RSD分别为1.2%、1.0%和1.3%。结论该方法快速稳定且分离度好,为桔梗的质量控制提供可借鉴的分析方法。

RP-HPLC determination of 3 compounds of triterpenoid saponins in Platycodon grandiflorum

Objective To develop an RP-HPLC method for the simultaneous determination of deapio-platycoside E,platycoside E,and platycodin D3 in Platycodon grandiflorum.Methods A Hypersil-ODS（4.6 mm×250 mm,5 μm） column was used with a mobile phase of acetonitrile（A）-water（B）.The flow rate was 0.8 mL·min-1,the UV detection was 210 nm,and the column temperature was room termperature.Results The linear ranges of the deapioplatycoside E,platycoside E,and platycodin D3 were 4.160-83.2,10.75-215.0,and 4.080-81.6 μg·mL-1,respectively,the average recoveries were 96.5%,97.0% and 97.3 %,respectively,and the RSDs were 1.2%,1.0% and 1.3%.Conclusion The method is reliable and it can be used for the quality control of Platycodon grandiflorum.