| 南蛇藤乙酸乙酯提取物诱导HepG2细胞凋亡的实验研究 |
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| 引用本文： | 熊熙,汪茂荣. 南蛇藤乙酸乙酯提取物诱导HepG2细胞凋亡的实验研究[J]. 实用肝脏病杂志, 2011, 14(4): 249-252. DOI: 10.3969/j.issn.1672-5069.2011.04.004 |
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| 作者姓名： | 熊熙 汪茂荣 |
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| 作者单位： | 1. 南京中医药大学第一临床医学院解放军第81医院全军肝病中心,南京市,210046
2. 解放军第81医院全军肝病中心 |
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| 基金项目： | 2008年南京军区"122工程"学科带头人培养基金 |
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| 摘 要： | 目的探讨南蛇藤乙酸乙酯提取物对HepG2细胞增殖和诱导凋亡的机制。方法采用CCK-8法检测细胞增殖;使用流式细胞仪检测细胞周期和细胞凋亡;采用分光光度计检测凋亡细胞胞浆caspase-3的活性变化。结果不同浓度的南蛇藤提取物能抑制HepG2细胞增殖,呈剂量效应和时间效应关系;药物作用24h时,其IC50为111.8μg/ml,作用48h时,其IC50为99.7μg/ml,作用72h时,其IC50为43.0μg/ml;药物干预24h时,HepG2细胞停滞在G0-G1期,并产生细胞凋亡亚二倍峰。在药物为15μg/ml、30μg/ml和60μg/ml时,细胞凋亡率分别为44.91%、31.95%和21.94%,与对照组比较,均有显著性差异（F=5.449,P〈0.05）;在药物浓度为30μg/ml、60μg/ml和120μg/ml并分别作用24h和48h时,Caspase-3活性逐渐增强。结论南蛇藤乙酸乙酯提取物抑制HepG2细胞增殖和诱导细胞凋亡的作用可能与其增强Caspase-3活性有关。
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| 关 键 词： | 南蛇藤 HepG2细胞 细胞增殖 细胞凋亡 Caspase-3 |
The inhibition of acetoacetate extractive of celastrus orbiculatus thunb on proliferation and apoptosis of HepG2 cells |
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| XIONG Xi,WANG Maorong. The inhibition of acetoacetate extractive of celastrus orbiculatus thunb on proliferation and apoptosis of HepG2 cells[J]. Journal of Clinical Hepatology, 2011, 14(4): 249-252. DOI: 10.3969/j.issn.1672-5069.2011.04.004 |
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| Authors: | XIONG Xi WANG Maorong |
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| Affiliation: | XIONG Xi,WANG Maorong.The First Clinical Medical College,Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China |
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| Abstract: | Objective To investigate the effects of acetoacetate extractive of celastrus orbiculatus thunb on HepG2 cells.Methods The proliferation of cells were tested with CCK-8;The cell cycles and the apoptosis of HepG2 cells intervened by acetoacetate extractive of celastrus orbiculatus thunb were detected by flow cytometry;The change of caspase-3 activity in HepG2 cells were detected by spectrophotometer.Results Acetoacetate extractive of celastrus orbiculatus thunb induced apoptosis of HepG2 cells in an obvious d... |
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| Keywords: | Caspase-3 |
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