头孢克肟分散片溶出度HPLC法与UV分光光度法测定结果的比较

目的：比较HPLC法与UV分光光度法测定头孢克肟分散片的溶出度值，并从中选择适宜的方法。方法：HPLC法采用KromasilC18色谱柱，流动相为10％四丁基氢氧化铵溶液一乙腈（72：28），流速为1mL／min，检测波长为254nlyl，柱温为40℃，进样量为20μL；UV分光光度法采用溶出介质为pH7．2磷酸盐缓；中液，检测波长为288nm，浆法，转速为50r／min。结果：2种测定方法测得的头孢克肟浓度在10．60～212．10μg／mL范围内均呈线性关系（r2=1）。结论：两种方法测得头孢克肟溶出度的结果基本一致。

Comparison of Dissolution of Cefixime Dispersible Tablets by HPLC and UV

Objective： To compare the dissolution of cefixime dispersible tablets by HPLC and UV methods, and the select an appropriate method. Methods： HPLC method： the determination was used Kromasil C18 column, 10% tet- rabuty lammonium hydroxide-acetonitrile（72：28） as the mobile phase at the flow rate of 1 mL/min.The detective wave- length was 254 nln, the column temperature was 40 ℃ and the injective volume was 20 μL. UV method： phosphate buf- fer（pH 7.2） was used as dissolution medium and the detection wavelength was at 288 nm. Apparatus was operated with the paddle method at the rate of 50 r/min. Results： A good linear relationship was obtained when the cefixime＇s concen- tration range is between 10.60 and 212.10 μg/mL（r2=l ）. Conclusion： Two methods are equivalent in determination of the dissolution of cefixime dispersible tablets.