化合物E-2-（3’，5’-二甲氧基苯亚甲基）-环己酮的体外抗肿瘤作用州

目的：研究化合物E-2-（3＇，5＇-二甲氧基苯亚甲基）-环己酮（IV8）对人类宫颈癌（HeLa）、肝癌（HepG2）、前列腺癌（Dul45）和胃癌（SGC7901）细胞系的抗肿瘤活性。方法：采用MTT法评价IV8对上述4株肿瘤细胞株增殖的影响，并在光学显微镜下观察lV8对HepG2细胞形态学上的变化，以及采用AO／EB双荧光染色法评价IV8对HepG2细胞的凋亡作用。结果：IV8抑制HeLa，HepG2，Dul45和SGC7901细胞的IC。值分别为2．10，11．32，36．50和89．53gmol／L；光学显微镜下观察IV8处理的细胞出现明显增殖抑制和形态学改变；AO／EB双荧光染色IV8组可见凋亡细胞。结论：药理实验结果表明IV8具有较好的体外抗肿瘤的活性，值得进一步研究。

Study on Anti-tumor Activities of 2-(3',5'-Dimethoxybenzylidene)Cyclohexanone in Vitro

Objective： To study the anti-tumor effects of 2-（3＇,5＇-dimethoxybenzylidene）-cyclohexanone（IV8） on human cancer cell lines （HeLa, Hep O2, Dul45 and SGC7901）. Methods： Compound IV8 was evaluated for antiprolif- erativc activities on the above cancer cell lines in vitro by MTT assay. The morphology alteration of Hep G2 was ob- served under inverted microscope and under AO/EB double staining. Results： Compound IV8 possessed significant in- hibito~ activity against HeLa, Hep G2, Du145 and SGC7901 with IC~ values of 2.10, 11.32,36.50 and 89.53 ~mol/L, respectively. Under the microscope, Hep G2 cells produced morphology alteration and cell number decreased signifi- cantly. AO/EB double staining found that IV8 group produced apoptotic cells.Conclusion： Preliminary pharmacological tests showed that IV8 had potent anti-tumor activities, which suggested that it was worthy of further studies.