促红细胞生成素影响骨髓间充质干细胞增殖的量效关系

背景：促红细胞生成素对骨髓间充质干细胞增殖有重要的作用,但剂量效应关系尚不明确。目的：观察不同剂量促红细胞生成素对兔骨髓间充质干细胞增殖活性与细胞周期的影响。方法：通过MTT比色试验、流式细胞仪FCM检测0,2,4,8,16U/mL不同浓度促红细胞生成素对兔骨髓间充质干细胞增殖周期的影响,同时收集培养液作基质金属蛋白酶2水平检测。结果与结论：随着加入促红细胞生成素浓度的增加,骨髓间充质干细胞增殖能力逐渐升高,以16U/mL组最高。加入2,4,8,16U/mL促红细胞生成素后,骨髓间充质干细胞S期比例及增殖指数均逐渐增高,增殖指数较未加入促红细胞生成素组增高（P〈0.05）;细胞培养液基质金属蛋白酶2水平却逐渐下降,以16U/mL组下降最明显。说明在2～16U/mL促红细胞生成素呈剂量依赖性促进骨髓间充质干细胞增殖力。

Dose-dependent effect of erythropoietin on the proliferation of rabbit bone marrow mesenchymal stem cells

BACKGROUND：Some studies have certificated that erythropoietin（EPO） can promote bone marrow mesenchymal stem cells（BMSCs） proliferation,but it is not yet clear about the dose-dependent relationship.OBJECTIVE：To explore the different-dose effects of EPO on rabbit BMSCs proliferation activity and cell cycle.METHODS：MTT colorimetric test and flow cytometry were used to detect the effect of EPO（0,2,4,8,16 U/mL） on BMSCs cell cycle;meantime,the culture medium was collected for matrix metalloproteinase-2（MMP-2） content detection.RESULTS AND CONCLUSION：2-16 U/mL EPO could promote BMSCs proliferation gradually in a dose-dependent manner.BMSCs percentage in S phase and proliferative index gradually increased with the dose increase of EPO,and the proliferation index of BMSCs with EPO was significantly higher than that of BMSCs without EPO（P 0.05）;however,the level of MMP-2 was decreased gradually,especially in 16 U/mL EPO group.These indicate that EPO can increase the proliferative ability of BMSCs in a dose-dependent manner.