蚯蚓纤溶酶活性影响因子的研究

目的 :对提取过程中影响蚯蚓纤溶酶活性的 5种因子进行了分析 ,以提高纤溶酶的提取活性。方法 :分别采用 0.9%NaCl抽提、10%蔗糖抽提、75%酒精抽提出粗酶。酶活测定方法采用尿激酶琼脂糖 纤维蛋白平板法。硒含量的测定方法采用 3,3′-二氨基联苯胺比色法 ;砷含量的测定方法采用二乙氨基二硫代甲酸银比色法。结果 :饲养在牛粪饵料中的蚯蚓纤溶酶活性比饲养于生活垃圾中的蚯蚓纤溶酶活性更高 ,蚯蚓体内砷的含量与纤溶酶活性呈正相关 ,而蚯蚓体内硒的含量对纤溶酶活性影响不大。在采用 3种不同的蚯蚓纤溶酶的抽提方法中 ,75 %酒精抽提效率最高 ,0.9%氯化钠次之 ,10%蔗糖最低 ;在蚯蚓纤溶酶纯化过程中 ,透析比超滤对蚯蚓纤溶酶的活性影响更小一些。结论 :通过将蚯蚓饲养在适宜的饵料中 ,使用合适的提取手段和提纯方法 ,可以提高蚯蚓纤溶酶的提取活性。

Factors Influencing the Activity of Fibrinolytic Enzymes From Earthworm, Eisenia foetida

OBJECTIVE: To study the factors influencing the activity of fibrinolytic enzymes from earthworm and to obtain the better way to extract fibrinolytic enzymes as well as keep its optimum activity. METHOD: 75% alcohol, 0.9% NaCl and 10% saccharose was used to extract the crude fibrinolytic enzymes from earthworm, the method of urokinase gelose-fibrin plate was used to measure the activity of fibrinolytic enzymes from earthworm. and the method of 3,3'-diaminobezidine tetrahydrochloride colorimetry to was used measure the content of selenium. The method use ts of measuring the content of arsenic was silver diethyldithiocarbamate colorimetry. RESULT: The fibrinolytin of earthworms reared with cattle soils had higher activity than that reared with garbage. The arsenic in the earthworm's body could improve the activity of earthworm's fibrinolytin. However, the selenium had litter influence on it. Among the three methods of extraction, the 75% alcohol one was the most efficient, the 0.9% NaCl was next, and the 10% saccharose was the lowest. The influence of dialysis on the activity of fibrinolytin was less than that of ultrafiltration, when the earthworm's fibrinolytin enzyme was further sublimated. CONCLUSION: The activity of the earthworm's fibrinolytin will be increased earthworm is reared with the fitting baits and when appropriate methods, of extraction and purification are used.