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Anti-hyperuricemic and nephroprotective effects of Modified Simiao Decoction in hyperuricemic mice
Authors:Hua Jian  Huang Ping  Zhu Chong-Mei  Yuan Xiao  Yu Chen-Huan
Institution:The Second People Hospital of Xihu District, Hangzhou 310024, China.
Abstract:

Ethnopharmacological relevance

Modified Simiao Decoction (MSD), based on clinical experience, has been used for decades and famous for its efficiency in treating hyperuricemic and gouty diseases.

Aim of the study

To investigate the effects of MSD on anti-hyperuricemic and nephroprotective effects are involved in potassium oxonate-induced hyperuricemic mice.

Materials and methods

The effects of MSD were investigated in hyperuricemic mice induced by potassium oxonate. MSD were fed to hyperuricemic mice daily at a dose of 0.45, 0.90, 1.80 g/kg for 10 days, and allopurinol (5 mg/kg) was given as a positive control. Serum and urine levels of uric acid and creatinine, and fractional excretion of uric acid (FEUA) were determined by colorimetric method. Its nephroprotective effects were evaluated by determining a panel of oxidative stress markers after the intervention in hyperuricemic mice. Simultaneously, protein levels of urate transporter 1 (URAT1) and organic anion transporter 1 (OAT1) in the kidney were analyzed by Western blotting.

Results

MSD could inhibit XOD activities in serum and liver, decrease levels of serum uric acid, serum creatinine and BUN, and increased levels of urine uric acid, urine creatinine, FEUA dose-dependently through down-regulation of URAT1 and up-regulation of OAT1 protein expressions in the renal tissue of hyperuricemic mice. It also effectively reversed oxonate-induced alterations on renal MDA levels and SOD activities in this model.

Conclusion

MSD processes uricosuric and nephroprotective actions by regulating renal urate transporters and enhancing antioxidant enzymes activities to improve renal dysfunction in hyperuricemic mice.
Keywords:MSD  Modified Simiao Decoction  TCM  traditional Chinese medicine  XOD  xanthine oxidase  BUN  blood urea nitrogen  SOD  superoxide dismutase  MDA  malondialdehyde  SUA  serum uric acid  SCr  serum creatinine  UUA  urinary UA  UCr  urinary creatinine  FEUA  fractional excretion of urate  URAT1  urate transporter 1  OAT1  organic anion transporter 1  NC  normal control  MC  model control  potassium oxonate-induced hyperuricemic mice  MC+AP  hyperuricemic mice treated with allopurinol at the dose of 5 mg/kg  MSD45  MSD90  MSD180  hyperuricemic mice treated with MSD at the dose of 0  45 g/kg0  90 g/kg  1  80 g/kg  respectively
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