| 经胶原包埋、修饰的聚羟基乙酸与软骨细胞体外培养的实验研究 |
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| 引用本文： | 吴利标,许建衡,刘婉秀,孙淑明,吴丽娥.经胶原包埋、修饰的聚羟基乙酸与软骨细胞体外培养的实验研究[J].中国美容医学,2002,11(6):517-518. |
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| 作者姓名： | 吴利标 许建衡 刘婉秀 孙淑明 吴丽娥 |
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| 作者单位： | 汕头大学医学院第一附属医院外科实验室,广东省汕头市,515041 |
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| 基金项目： | 广东省医学科研基金资助项目(A199703) |
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| 摘 要： | 目的：探讨经胶原包埋、修饰后的聚羟基乙酸（poloyglycolic acid简称PGA）作为组织技术中细胞培养支架的可行性；方法：将用胶原包埋的PGA和没有包埋的PGA分别与软骨细胞共同置于二氧化碳培养箱中培养，观察二者的亲水性，对细胞的吸附能力和细胞分泌基质的能力进行比较；结果：以胶原包埋的PGA和没有包埋的PGA亲水性没有明显的差异，二者亲水性均不强，而前者对细胞的吸附能力和细胞在其上面生长、分泌基质的能力明显增强；结论：以胶原包埋、修饰的PGA作为组织工程技术中细胞生长的支架，具有很大的应用前景。
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| 关 键 词： | 胶原包埋 胶原修饰 聚羟基乙酸 软骨细胞 体外培养 实验研究 |
| 文章编号： | 1008-6455(2002)06-0517-02 |
| 修稿时间： | 2002年3月26日 |
THE EXPERIMENTAL STUDY ON CULTURE OF CHONDROCYTES SEEDED ONTO POLOLYGLYCOLIC ACID SCAFFOLDINGS COATING WITH COLLAGEN |
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| WU Li -biao,XU Jian -heng,LIU Wan -xiu,SUN Shu -ming and WU Li -e.THE EXPERIMENTAL STUDY ON CULTURE OF CHONDROCYTES SEEDED ONTO POLOLYGLYCOLIC ACID SCAFFOLDINGS COATING WITH COLLAGEN[J].Chinese Journal of Aesthetic Medicine,2002,11(6):517-518. |
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| Authors: | WU Li -biao XU Jian -heng LIU Wan -xiu SUN Shu -ming and WU Li -e |
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| Abstract: | Objective:To find the possibiltly of poloyglycolic acid(PGA)coating with collagen used as cell sc affold in tissue engi-neering.Methods:T he chondrocytes seeded onto PGA and P GA coating with collagen were cultur ed in CO 2 culturing box.With light microscope and scanning electron mi croscope the scaffolds in hydrophil ia and adsorptivity to chondrocytes and the function of the cell were observed.Results:The hydrophidia of PGA and PGA coatin g with collagen are all weak but no different in them.The chondrocytes were seeds onto PGA with collagen were str onger in growth than that onto PGA and secretory matrixes.Conclusion:The PGA coated with collagen will be used widely as scaffolds in tissue engineering in the future |
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| Keywords: | cell scaffold poloyglycolic acid(PGA) experimental study |
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