长链非编码RNA-HOST2在上皮性卵巢癌中的表达及生物学功能

目的:分析长链非编码RNA-HOST2在上皮性卵巢癌细胞和组织中的表达,探讨HOST2表达与卵巢癌生物学行为之间的潜在关系,为上皮性卵巢癌的靶向治疗提供理论依据和实验数据。方法:选择OVCAR-3上皮性卵巢癌细胞株,用含10%胎牛血清的RPMI 1640培养基常规标准条件下培养,利用实时定量荧光PCR技术检测HOST2的表达,利用siRNA靶向干扰HOST2表达,利用划痕实验、Transwell细胞小室和CCK-8试剂研究HOST2对上皮性卵巢癌细胞迁移、侵袭、增殖和凋亡的影响。结果:HOST2在上皮性卵巢癌中均特异性高表达,但在其他多种常见的恶性肿瘤中表达很低或几乎没有表达。HOST2的表达受抑制后,OVCAR-3细胞的迁移、侵袭和增殖能力均明显减弱,外源性降低HOST2表达能抑制上皮性卵巢癌皮下移植瘤的生长,延长裸鼠生存期。结论:HOST2参与上皮性卵巢癌细胞的迁移、侵袭、增殖等生物学行为,可用于上皮性卵巢癌的靶向治疗。

Expression and function of long non-coding RNA HOST2 in epithelial ovarian cancer

Objective:To verify the specific expression of HOST2 in epithelial ovarian cancer and test whether HOST2 contribute to biological functions in order to provide theoretical foundation and experimental data,and finially may identify a therapy target of epithelial ovarian cancer.Methods:OVCAR-3cell line,cultured by 10% FBS RPMI-1640 media,was used to be cytological subjects.mRNA expression was evaluated by quantitative real-time polymerase chain reaction,and protein expression by Western blot.Changing endogenous level of HOST2 by liposome transfection,and then test the effects on biological behaviours of tumor cells.Results:HOST2specifically high expresses in human epithelial ovarian cancer,but barely expresses in other kinds of solid malignancies.HOST2 participates in migration,invasion and proliferation of ovarian cancer cells.Conclusions:HOST2high expression acts as cancer-specific events that may be contributed to the new biomarker of epithelial ovarian cancer,and therapeutic approaches for patients.