| 牙囊细胞与纳米晶羟基磷灰石/胶原复合材料体外相容性研究 |
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| 引用本文： | 叶国,董伟,吴亚菲,付钢.牙囊细胞与纳米晶羟基磷灰石/胶原复合材料体外相容性研究[J].重庆医学,2009,38(17). |
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| 作者姓名： | 叶国 董伟 吴亚菲 付钢 |
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| 作者单位： | 1. 重庆医科大学附属口腔医院,400015
2. 四川大学华西口腔医院牙周科,成都,610041 |
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| 摘 要： | 目的 探讨大鼠牙囊细胞(DFCs)与纳米晶羟基磷灰石/胶原复合材料(nHAC)的相容性,为二者在牙周组织工程中的应用提供依据.方法 将大鼠DFCs与nHAC在体外复合培养,通过倒置显微镜、扫描电镜观察细胞与材料的复合情况,用MTT法、碱性磷酸酶活性检测法检测材料对细胞增殖分化的影响.结果 DFCs可以在nHAC材料表面及孔隙中良好的黏附、增殖和分化,复合培养5d后nHAC对DFCs的增殖分化表现出一定的促进作用.结论 DFCs与nHAC具有良好的生物相容性.
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| 关 键 词： | 牙囊细胞 纳米羟基磷灰石/胶原复合材料 生物相容性 |
Cellular compatibilty of dental follicle cells and nano-hydroxyapatite collagen |
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| YE Guo,DONG Wei,WU Ya-fei,et al..Cellular compatibilty of dental follicle cells and nano-hydroxyapatite collagen[J].Chongqing Medical Journal,2009,38(17). |
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| Authors: | YE Guo DONG Wei WU Ya-fei |
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| Institution: | YE Guo1,DONG Wei2,WU Ya-fei2,et al.(1.Affiliated Hospital of Stomatology,Chongqing Medical University,Chongqing 400015,China,2.West China School of Stomatology,Sichuan University,Chengdu 610041,China) |
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| Abstract: | Objective To assess the compatibility of dental follicle cells and nano-hydroxyapatite collagen.Methods Cultivated the compound of rat DFCs and nHAC in vitro;observed the compound situation through inverted microscope,SEM;tested the cell multiplication and differentiation under the influence of the material by MTT and ALP methods.Results DFCs had good qualities of adherency,multiplication and differentiation on the surface and in the hole of nHAC.After 5d,nHAC accelerated DFCs multiplication and differentia... |
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| Keywords: | dental follicle cells nano-hydroxyapatite collagen biocompatibility |
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