四物汤促进造血功能成分的初步研究

目的 :初步探讨四物汤补血作用的物质基础。方法 :四物汤部位分离采用醇沉和萃取法。用辐射小鼠血虚证模型考察活性。结果 :首先将四物汤分离为A ,B ,C 3个部位 ,活性研究表明部位B和部位C具有提高模型小鼠外周血白细胞数的作用。进而将部位B和部位C分别分离为部位B1,B2 ,B3和部位C1,C2 ,C3,活性研究表明部位C2 ,C3和B3具有提高模型小鼠外周血白细胞数的作用。进一步考察部位C1,C2 ,C3,B和芍药苷对模型小鼠骨髓造血祖细胞的影响 ,结果表明部位C2 ,C3,B和芍药苷具有促进模型小鼠骨髓造血祖细胞增殖的作用。结论 :四物汤部位C2 ,C3,B和芍药苷与其促进造血功能的作用有关。

Preliminary study on hematopoietic constituents of SI-WU-TANG

Objective: To make preliminary study on the chemical foundation of hematopoietic effect of SI-WU-TANG. Method: SI-WU-TANG was fractionated by using precipitation method with ethanol and liquid-liquid extraction. Activity was investigated on radiated mice as model of blood deficiency. Result: At first SI-WU-TANG was separated into three fractions,fraction A,B and C. Activity study showed that fraction B and C could raise the amount of peripheral white blood cell in radiated mice. Then fraction B and C were respectively further separated into fraction B1,B2,B3 and fraction C1,C2,C3. Activity study showed that fraction C2,C3 and B3 could raise the amount of peripheral white blood cell in radiated mice. Furthermore,effect of fraction C1,C2,C3,B and paeoniflorin on hematopoietic progenitor cell in bone marrow of radiated mice were investigated. Fraction C2,C3,B and paeoniflorin could promote the proliferation of hematopoietic progenitor cell in bone marrow of radiated mice. Conclusion: Fraction C2,C3,B and paeoniflorin are responsible for hematopoietic activity of SI-WU-TANG.