胚肝细胞中树突状细胞的分离、培养和表型鉴定

本实验主要以胚肝细胞作为细胞来源，通过贴壁处理，分离DCs的前体细胞，在含细胞因子的培养体系中培养，诱导其分化为树突状细胞。比较不同处理和不同来源细胞所得到的树突状细胞生长状况、细胞表型和异同、细胞功能，以寻找一种体外大量扩增人树突状细胞的新方法．

Induction and Phenotypical Analysis of Dendritic Cells from Fetal Livers

Dendritic cells(DCs)is the most important subset of Antigen Present Cells (APCs )in vivo. The important function of DCs is to stimulate cytotoxic T cells to kill tumor cells. Many studies have been performed to look for a method for inducing, proliferating and purifying DCs in the last few years. In thisstudy, we isolated the progenitor of DCs from fetal livers and the others afer their adhesion, and then putthem into the culture system containing the cytokines in order to induce them into DCs. As a result, thephenotypical and proliferative difference between DCs from different source(fetal liver of cord blood)afterdifferent treatment was found during the morphological observation and the cell phonetype analysis. In addition, we tested the function of DCs so that a practical method for getting a large number and highly purified dendritic cells has been developed.