肺炎克雷伯菌（ESBLs）氨基糖苷类修饰酶基因的研究

目的了解临床分离的产超广谱β-内酰胺酶（ESBLs）的肺炎克雷伯菌的耐药性及氨基糖苷类修饰酶基因的存在状况。方法对临床分离的35株产ESBLs肺炎克雷伯菌采用聚合酶链反应技术（PCR）检测6种氨基糖苷类修饰酶aac（3）-Ⅰ，aac（3）-Ⅱ，aac（6）-Ⅰ，age（6’）-Ⅱ，ant（3″）-Ⅰ，ant（2″）-Ⅰ。结果该35株肺炎克雷伯菌检出：aac（3）-Ⅰ阳性4株（11．8％），aac（3）-Ⅱ阳性7株（20．6％），aac（6’）-Ⅰ阳性13株（37．1％），aac（6’）-Ⅱ阳性7株（20％），ant（3″）-Ⅰ阳性11株（31．4％），ant（2″）-Ⅰ阳性12株（34.3％）。共有26株（74．3％）检出AMEs。同一菌株可产生两种或两种以上的氨基糖苷类修饰酶基因。结论临床分离的产ESBLs肺炎克雷伯茵氨基糖苷类修饰酶基因携带率高。

Study on aminoglycosides-modifying enzymes gene of extended-spectrum beta-lactamases (ESBLs)-producing Klebsiella pneumoniae

Objective To study the antimicrobial resistance and genotyping ofaminoglycosides-modifying enzymes （AMEs） in ESBLs-producing Klebsiella preumoniae （KPN） isolated from hosipital-acquired infection. Methods Six A/VIEs genotypes of KPN were verified by polymerase chain reaction （PCR）. Results PCR shows that ： 26 strains of KPN （74.3%） could be found one or more type of AMEs gene.The positive rate of six AMEs gene： aac（3）- Ⅰ ,aac（3）- Ⅱ ,aac（6＇）- Ⅰ ,aac （6＇）- Ⅱ ,ant（3＂） - Ⅰ ,ant（2＂）- Ⅰ were 11.8%, 20.6%,37.1%, 20%,31.4%,34.3%. Two or more AMEs were produced by the same bacilli. Conclusion There is high positive percentages of AMEs gene in the ESBLs-producing Klebsiella pneumoniae.