Induction of IFN-{gamma} in macrophages by lipopolysaccharide |
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Authors: | Fultz Marion J; Barber Sheila A; Dieffenbach Carl W; Vogel Stefanie N |
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Institution: | Department of Microbiology, Uniformed Services University of the Health Sciences 4301 Jones Bridge Road, Bethesda, MD 20814, USA
1 Division of AIDS NIAID, NIH, Bethesda, MD 20892, USA |
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Abstract: | In this paper we report that macrophages can be stimulated toexpress detectable levels of IFN--specific mRNA. Macrophagesfrom lipopolysaccharide (LPS)-responsive, C3H/OuJ mice are inducedby LPS to increase steady-state levels of IFN--specific mRNA,while those from LPS-hyporesponsive C3H/HeJ mice are not. Thisinterstrain variation is apparently the result of LPS-specificsignal differences since macrophages derived from both Lpsnand Lpsd mouse strains are able to produce comparable levelsof IFN--specific mRNA following stimulation with polyinosinic-polycytidylicacid. The identity of the cell type responsible for this IFN-message appears to be the macrophage as IFN--specific mRNA wasalso detectable following T and natural killer cell depletion,in the LPS-stimulated RAW 264.7 cell line, and in a homogeneouspopulation of mature macrophages propagated in vitro by stimulationof bone marrow progenitors with recombinant colony stimulatingfactor-1. Immunofluorescent staining of fixed and permeabilizedLPS-stimulated macrophages confirmed the presence of immunoreactiveIFN- protein. The possible significance of IFN- production bymacrophages is discussed in the context of normal macrophagedifferentiation as well as the inflammatory immune response. |
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Keywords: | C3H/HeJ endotoxin polyinosinic-polycytidylic acid |
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