Dishevelled2和Vangl2蛋白在小鼠腭板发生中的表达及意义

目的 探讨Dishevelled2和Vangl2蛋白与小鼠腭发育的关系。 方法 将24只孕鼠随机分为8组，每组3只，分别于孕13d上午8时(p13d 8h，以下同）、p13d14h、p13d22h、p14d8h、p14d14h、p14d22h、p15d8h和p15d22h 8个时间点剖宫取胚鼠，常规石蜡切片，采用免疫组织化学及图像分析方法，检测小鼠发生各个时期腭板中Dishevelled2和Vangl2蛋白的分布状况和变化规律。 结果 两种蛋白在不同发育阶段的小鼠腭板上皮和间充质中均有不同程度的表达。Dishevelled2在腭板上皮和间充质均呈现先上升（p13d8h～p13d22h），而后下降（p13d22h～p14d14h），之后再次上升（p14d14h～p15d22h）的趋势；Vangle2在腭板间充质也呈现先上升（p13d8h～p13d22h），后下降（p13d22h～p14d22h），随后再上升（p14d22h～p15d22h）的趋势，其在上皮中的表达无明显规律。两种蛋白在腭板上皮的表达水平均明显高于其同期间充质的表达水平。 结论 Dishevelled2蛋白和Vangl2蛋白可能直接或间接地参与小鼠腭板的形态发生过程的调节，并且在此过程中两种蛋白均发生着规律性的时空变化。

Expression and its significance of Dishevelled2 and Vangl2 proteins in the developing mouse palate

Objective To explore the relationship between the expression of Dishevelled2 and Vangl2 proteins and the development of mouse palate. Methods Twenty-four pregnant mice were randomly divided into eight groups, and the mouse embryos were obtained at eight clock of the pregnant day of thirteen(p13d8h), p13d14h,p13d22h,p14d8h,p14d14h,p14d22h,p15d8h and p15d22h respectively, then paraffin sections were made conventionally.The distrubution and dynamic changes of Dishevelled2 and Vangl2 proteins in the embryonic palatal shelves were detected by immunohistochemistry and image analysis. Results It was found that the two kinds of proteins expressed in the epithelium and mesenchyma of the mouse palatal shelves at different development stages. The expression levels of the Dishevelled2,in both of the epithelium and mesenchyme of the palatal shelves, increased first (p13d8h-p13d22h),then decreased rapidly(p13d22h-p14d14h), and then increased again(p14d14h-p15d22h). The expression of Vangl2 protein in the mesenchyma showed a similar trend to that of the Dishevelled2, but there was no obvious regularity in the epithelium. In addition, the expressive levels of both kinds of proteins in the epithelium were significantly higher than those in mesenchyma of the palatal shelves. Conclusion Dishevelled2 and Vangl2 proteins might directly or indirectly take part in the regulation process of mouse palate morphogenesis.