脱细胞同种异体神经移植物的制备及成分分析

目的　探讨脱细胞同种异体神经移植的制备方法及其含有的成分。方法　采用组织工程化低渗脱细胞方法制备神经异体移植物 ,光电镜观察其结构特征 ;用免疫组织化学法和聚丙烯酰胺凝胶电泳法分析神经异体移植物的成分。结果　正常的 (天然的 )周围神经经脱细胞处理后 ,清除了雪旺细胞、神经外膜和束膜的细胞 ,以及神经纤维的髓鞘和轴突 ,保存了由雪旺细胞基底膜管以及神经外膜和束膜的细胞外基质构成的三维支架结构。成分分析结果显示 ,含有LN、FN以及生长相关蛋白GAP 4 3和 6 5kDa蛋白等促进和诱导受体损伤神经再生的重要成分。结论　脱细胞异体神经移植物含有诱导和促进神经再生的相关蛋白 ,有利于受损神经的再生。

Preparation of Acellular Nerve Allografts and Analysis of Their Components

Objective To study the preparation of the acellular nerve allografts and analyze the components. Methods The acellular nerve allografts were prepared with the hypotonic acellular method and the characters of the structure were observed under light and electron microscope, and the components were analyzed by immunohistochemistry and polyacrylamidedel electrophoresis (PAGE).Results Schwann cells, the cells of epineurium or nerve fascicles, myelin sheath and axon of nerve fibers were eliminated, but the basement membrane tube of Schwann cells and three-dimensional supports of extracellular matrix of epineurium and nerve fascicles were reserved for normal peripheral nerves treated acellularly. The component analytical results of the acellular nerve allografts indicate that they contain laminin, fbronectin, growth associated protein GAO-43 and 65kDa protein those can facilitate and induce the regeneration of the injured nerves.Conclusion The acellular nerve allografts contain several, proteins those induce and facilitate the regeneration of the injured nerves.