参麦、血必净影响家兔严重烫伤早期血管内皮细胞的研究

目的:建立严重烫伤家兔模型,观察家兔严重烫伤早期血管内皮细胞(vascular endothelial cell, VEC)功能变化及参麦、血必净注射液对功能的影响,并探讨它们对血管内皮细胞功能作用的相关机制,同时对两药疗效进行比较.方法:将家兔分成假伤组(8只)、烫伤组(8只)、参麦组(8只)和血必净组(8组);后3组制成30%体表总面积(TBSA)Ⅲ度烫伤(用92℃水浴烫伤)模型,假伤组采用37℃水浴模拟致伤过程;伤后各组按Parkland公式计算液体量予以复苏,同时参麦组每次给予参麦注射液2ml/kg加入等渗盐水至20ml静脉滴注,每日3次,连续2天;血必净组每次给予血必净注射液2ml/kg加入等渗盐水至20ml静脉滴注,每日3次,连续2天;另2组同法输注等容量等渗盐水.在伤后即刻、6小时、12小时、24小时、48小时时相点抽取家兔颈静脉血检测内皮素-1、一氧化氮并计算两者比值水平,同时观察相应时相点家兔的一般情况.结果:在伤后48小时, 假伤组、参麦组、血必净组ET-1/NO明显小于烫伤组;参麦组、血必净组血清ET-1和NO水平均明显低于烫伤组(P＜0.01);参麦组、血必净组之间比较无统计学意义(P>0.05);参麦组、血必净组家兔活跃程度明显优于烫伤组.结论:参麦注射液和血必净注射液可能是通过降低严重烫伤早期家兔血清ET-1和NO水平,调整ET-1/NO比值,对血管内皮细胞起保护作用.

Study of the Influence of Shenmai Injection and Xuebijng Injection on Vascular Endothelial Cell of Rabbit with Severe Burn at Early Stage

Objective： To establish a rabbit model of severe burns and to observe the influence of Shenmai injection and Xuebijing injection on vascular endothelial cell （ vascular endothelial cell, VEC） in rabbit with severe burn at early stage. On the basis of above study, relevant mechanisms of two drugs＇ function on VEC were discussed and the effects of two drugs were compared. Methods： Rabbits were divided into sham injury group （n = 8）, scald group （n = 8）, Shenmai group （ n = 8 ） and Xuebijing group （ n = 8 ） . The burn models of Ⅲ degree scald with 30% TBSA （ caused by 92 ℃ water bath scald） were established in later 3 groups. The rabbits in sham injury group were simulated injury with 37 ℃ water bath. After injury, each group was treated with fluid resuscitation according to Parkland formula, while the rabbits in Shenmai group were given intravenous infusion with the solution of 2ml/kg of Shenmai diluted to 20ml with isotonic saline. The infusion was conducted 3 times a day for 2 days; in Xuebijing group, the rabbits were given intravenous infusion with the solution of 2m/kg of Xuebijing diluted to 20ml with isotonic saline, 3 times a day for 2 days ; in the rest other two groups, the rabbits were treated with intravenous infusion of the same volume of isotonic saline only. The content of ET - 1 and NO of the sampling carotid blood of the rabbits were determinated and the value of ET - 1/NO was calculated at the time point of 0, 6, 12, 24 and 48h post - injury. Meanwhile, the general conditions of the rabbits at the corresponding time point were observed and recorded. Results： At the time point of 48th h post - injury, ET - 1 / NO ratio of rabbits in sham injury group, Shenmai group and Xuebijng group was obviously less than that in scald group. The level of ET - 1 and NO in Shenmai group and Xuebijng group were significantly lower than that of scald group （P 〈 0.01 ） . But there was no significant difference of level of ET - 1 and NO observed between Shenmai group and Xuebijng group （ P 〉 0. 05 ） . The degree of rabbit＇s activity in Shenmai group and Xuebijing group was better than that of scald group. Conclusions ： The protective role of Shenmai injection and Xuebijing injection on VEC may be by means of reducing the serum levels of ET - 1 and NO, and adjusting ET - 1 / NO ratio in severe scald rabbits at the early stage.