登革病毒感染对T细胞和NK细胞表达细胞因子的影响

【目的】 本文探讨登革病毒体外感染人外周血单个核细胞后对T细胞和NK细胞表达细胞因子的影响,并初步探讨登革病毒感染引起的免疫效应。【方法】用登革2型病毒(DEN-2)感染健康人外周血单个核细胞(PBMC),流式细胞术检测登革2型病毒后T细胞和NK细胞的表型及细胞因子的表达水平。【结果】 DEN-2感染18 h后,T细胞明显表达CD69、IFN-γ、TNF-α和perforin,NK细胞表达CD69、IFN-γ和perforin;随着感染复数(MOI)增加,表达perforin和IFN-γ的T细胞数及表达perforin的NK细胞数上升;随着感染时间的延长,表达perforin和IL-4的T细胞数及表达perforin的NK细胞数逐渐上升,而表达IFN-γ的T细胞数及表达IFN-γ的NK细胞数逐渐下降;表达perforin的T细胞亚型主要为CD8+T细胞。【结论】 发现登革病毒在体外能够诱导T细胞表达CD69、IFN-γ、TNF-α、perforin和IL-4,及 NK细胞表达CD69、IFN-γ和perforin,提示T细胞、NK细胞可能在抗登革病毒感染或免疫损伤中起着重要的作用。

Effects of Dengue Virus Infection on Expression of Cytokines in T cells and NK Cells

【Objective】 To investigate the expression of cytokines in T cells and NK cells from human peripheral blood mononuclear cells in response to dengue virus in vitro,and to explore the immune response after dengue virus infection.【Methods】 Flow cytometry was used to detect the phenotype and cytokines of T cells and NK cells from PBMC after DEN-2 infection.【Results】 T cells express CD69,IFN-γ,TNF-α,perforin,and NK cells express CD69,IFN-γ,perforin 18 hours after DEN-2 infection.The numbers of IFN-γ and perforin expressing T cells and perforin expressing NK cells have a positive correlation with dengue virus dose.The numbers of perforin and IL-4 expressing T cells and perforin expressing NK cells increase,whereas the numbers of IFN-γ expressing T cells and NK cells decrease follow infection times.T cells expressing perforin are mainly CD8+T cells.【Conclusion】 With in vitro infection by dengue virus,T cells can express CD69,IFN-γ,TNF-α,perforin and IL-4.And NK cells can express IFN-γ and perforin at the same time.We can conclude that T and NK cells may play an important role in the anti-dengue virus infection or immunopathology.