支气管哮喘患者血清白细胞介素35水平变化及意义

【目的】 探讨支气管哮喘患者血清白细胞介素35水平变化及意义。【方法】 将45例受试者分为支气管哮喘组(25例)和健康对照组(20例),测定支气管哮喘组患者未控制、部分控制后1周、控制后1周的血清白细胞介素35(IL-35)水平及各项主要肺功能指标包括第一秒用力呼气容积(FEV1)与预计值的比值?用力肺活量(FVC)与预计值的比值、FEV1与FVC的比值、呼气峰流量(PEF)与预计值的比值,以及未控制阶段的FEV1下降20%的吸入药物累积浓度(PC20-FEV1),测定健康对照组血清IL-35水平,分析哮喘患者与健康人血清IL-35水平的差异及其与哮喘控制水平、气道反应性的相关性。【结果】 支气管哮喘组未控制、部分控制以及控制阶段血清IL-35浓度(pg/mL)分别为141 ± 45、260 ± 84、366 ± 94,健康对照组血清IL-35浓度(pg/mL)为396 ± 120,支气管哮喘组未控制、部分控制阶段血清IL-35水平均低于健康对照组(P值均小于0.001),支气管哮喘组控制阶段与健康对照组血清IL-35水平差异无统计学意义(P = 0.36)。血清IL-35水平在未控制阶段低于部分控制阶段(95%置信区间为-163.10 ~ -73.65,P < 0.001),部分控制阶段低于控制阶段(95%置信区间为-151.41 ~ -61.97,P < 0.001)。哮喘患者血清IL-35水平与哮喘控制水平呈正相关,与PC20-FEV1无相关性。【结论】 健康人与哮喘患者血清中均存在IL-35表达,血清IL-35水平可能可以作为哮喘控制水平评估的免疫学参考指标。

Change of Serum Interleukin 35 Concentration in Asthma and Its Significance

【Objective】 To explore changes and significance of serum interleukin-35 concentration in patients with bronchial asthma.【Method】 Forty-five subjects were divided into two groups: bronchial asthma group with 25 patients and control group with 20 healthy subjects.To detect serum IL-35 concentrations of those uncontrolled,partly controlled and controlled after a week in the asthma group and their main pulmonary function indicators(including FEV1/FEV1 predicted value,FVC/FVC predicted value,FEV1/FVC,PEF/PEF predicted value) and PC20-FEV1 during uncontrolled period,and detect the serum IL-35 concentration of control group,and then analyze the difference of serum IL-35 concentration between both groups and the relevance of serum IL-35 concentration with asthma controlled level and airway reactivity.【Result】 Serum IL-35 concentrations(pg/mL) during uncontrolled period,partly controlled period and controlled period were 141 ± 45,260 ± 84,366 ± 94.Serum IL-35 concentration(pg/mL) in control group was 396 ± 120.Serum IL-35 concentrations of asthma group during uncontrolled and partly controlled periods are lower than control group(t were respectively-9.427 and-4.458,P < 0.001).There exists no significance difference between serum IL-35 concentrations in asthma group during controlled period and control group(P = 0.36).Serum IL-35 concentration during uncontrolled period is lower than that during partly controlled period(95% Confidence Interval:-163.10～-73.65,P < 0.001),and serum IL-35 concentration during partly controlled period is lower than that during controlled period(95% Confidence Interval:-151.41 ～-61.97,P < 0.001).IL-35 level of asthma patients shows positive correlation with asthma controlled level and shows no correlation with PC20-FEV1.【Conclusion】 There expresses IL-35 in serum of asthma patients and healthy persons.Serum IL-35 concentration may be used as index to test asthma controlled level.