原发性肝癌术后早期复发密切相关microRNA的筛选及应用

【目的】 利用microRNA芯片技术分析比较两组不同复发倾向肝癌患者的miRNA表达谱,并在更大样品的基础上进行实验验证?【方法】收集10例原发性肝癌组织标本根据术后复发情况分2组:早期复发组与非早期复发组,运用microRNA芯片技术筛选出差异表达显著的microRNAs?结合文献报道,选择mir-144?mir-502-3p作为其他82例肝癌组织标本qReal-time PCR验证的检测指标?并利用生物信息学方法预测其靶基因?【结果】本研究共筛选获得7个差异表达的microRNAs?与非早期复发组相比,在早期复发组中有4个表达上调,3个表达下调?qReal-time PCR验证与microRNA芯片筛选的结果一致?生物信息学方法预测miR-144靶基因可能为Rb1,miR-502-3p靶基因可能为SET?【结论】 microRNA芯片筛选的mir-144?mir-451?mir-486-5p?mir-602?mir-551b?mir-96?mir-502-3p与原发性肝癌术后早期复发密切相关?

Screening and Validating MicroRNA Associated with Early Recurrence after Radical Surgery in Primary Hepatocellular Carcinoma

【Objective】 To select effectiveness of microRNAs and correlated with early recurrence after radical surgery in liver cancer by microRNAprofiling, which analyzing verified the results by tissue samples on the basis. 【Methods】 10 HCC cases with radical surgery were divided into 2 group: early recurrence group and non-early recurrence group, according to postoperative recurrence in primary hepatocellular carcinoma. MicroRNA profiling study were performed to obtain some different and significant microRNAs. According to literature, mir-144 and mir-502-3p were used to detect HCC recurrence by real-time PCR with 82 HCC cases, and target genes were predicted using bioinformatics. 【Results】 Expression of 7 miRNAs were different and significant in the HCC with early recurrence group. Comparing with non-early recurrrence group, 4 miRNAs were up-regulated in early recurrence group. However, 3 miRNAs were down-regulated. The result of mir-144 and mir-502-3p expression by real-time PCR was uniformity to microarray. Rb1 might be a potential target gene of mir-144; SET might be a potential target gene of mir-502-3p. 【Conclusions】 Mir-144, mir-451, mir-486-5p, mir-602, mir-551b, mir-96, and mir-502-3pwere correlated with early recurrence after radical surgery in primary hepatocellular carcinoma with analyzing and screening of microRNA profile by microarray.