人骨髓间充质干细胞与脐血CD34+细胞体外扩增

为了研究骨髓间充质干细胞(MSC)及细胞因子对脐血CD34 造血祖细胞体外扩增的作用,及其扩增作用 对细胞黏附分子的影响,用免疫磁珠富集脐血CD34 细胞,然后接种到含有或不含有MSC和细胞因子的24孔培 养板,体外培养1周,观察不同指标并进行组间比较。结果表明:①SDF-1α SCF TPO FL因子组合与SCF TPO FL因子组合对脐血CD34 细胞的扩增作用无显著性差异(无论有无MSC细胞层存在)(P>0.05);②MSC 与上述细胞因子共存的培养体系优于相应的单纯细胞因子培养体系(P<0.05);③扩增前与扩增后脐血造血祖 细胞黏附分子CD44的表达没有明显变化。结论:趋化因子SDF-1α对SCF TPO FL因子组合的扩增作用无显 著影响;MSC增加细胞因子的脐血细胞体外扩增的作用;体外扩增不影响跻血细胞黏附分子CD44的表达。

Expansion ex vivo of Human Bone Marrow Mesenchymal Stem Cells and Cord Blood CD34 + Cells

The aim was to study the roles that the bone marrow mesenchymal stem cells (MSC) and cytokines play in cord blood CD34(+) cell expansion ex vivo and the influence of culture ex vivo on expression of the adhesive molecule of CD44. CD34(+) cells sorted from cord blood cells had been cultured in each well of 24 well culture plates containing culture medium supplemented with mesenchymal stem cells layer or/and cytokines for a week, and then all kinds of indexes of different groups were compared. The results showed that as for cord blood cell expansion, there was no significant difference between the groups with cytokines SDF-1alpha + SCF + TPO + FL and SCF + TPO + FL no matter if MSC layer existed or not. The groups with MSC layer and cytokines were superior to the corresponding groups without MSC layer. In addition, the expression of the adhesion molecule CD44 had no distinct change after culture. It is concluded that SDF-1alpha has no distinct influence on the effect of cytokines SCF + TPO + FL on cord blood cell expansion ex vivo. MSC enhance the effect of cytokines on cord blood cell expansion ex vivo. Such expansion ex vivo may not influence the expression of the adhesive molecule CD44 on cord blood cells.