Virally-directed fluorescent imaging (VFI) can facilitate endoscopic staging |
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Authors: | P S Adusumilli D P Eisenberg B M Stiles K J Hendershott S F Stanziale M-K Chan M Hezel R Huq V W Rusch Y Fong |
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Institution: | (1) Department of Surgery, Memorial Sloan–Kettering Cancer Center, New York, NY 10021, USA;(2) Molecular Cytology Core Facility, Memorial Sloan–Kettering Cancer Center, New York, NY 10021, USA |
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Abstract: | Background Replication-competent, tumor specific herpes simplex virus NV1066 expresses green fluorescent protein (GFP) in infected cancer
cells. We sought to determine the feasibility of GFP-guided imaging technology in the intraoperative detection of small tumor
nodules.
Methods Human cancer cell lines were infected with NV1066 at multiplicities of infection of 0.01, 0.1 and 1. Cancer cell specific
infectivity, vector spread and GFP signal intensity were measured by flow cytometry and time-lapse digital imaging (in vitro); and by use of a stereomicroscope and endoscope equipped with a fluorescent filter (in vivo).
Results NV1066 infected all cancer cell lines and expressed GFP at all MOIs. GFP signal was significantly higher than the autofluorescence
of normal cells. One single dose of NV1066 spread within and across body cavities and selectively infected tumor nodules sparing
normal tissue. Tumor nodules undetectable by conventional thoracoscopy and laparoscopy were identified by GFP fluorescence.
Conclusion Virally-directed fluorescent imaging (VFI) is a real-time novel molecular imaging technology that has the potential to enhance
the intraoperative detection of endoluminal or endocavitary tumor nodules.
R. Huq: Molecular Cytology Core Facility
Presented at the Society of American Gastrointestinal and Endoscopic Surgeons (SAGES) meeting, Fort Lauderdale, FL, USA, 13–16
April 2005 |
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Keywords: | Fluorescent detection Oncolytic virus Gene therapy Fluorescent laparoscopy Fluorescent thoracoscopy Herpes simplex virus |
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