浙江省在鼠形动物中检测到伯氏疏螺旋体

目的了解鼠形动物中自然感染伯氏疏螺旋体的状况。方法用巢式聚合酶链反应扩增鼠中的伯氏疏螺旋体5S--23SrRNA间隔区片段，对阳性产物进行测序。结果在浙江省金华市金东区捕获鼠形动物128只，在黄毛鼠、姬鼠、臭鼬鼯中共检测到阳性12份，阳性率分别为9．68％、14．28％和25．00％，并对其中2份进行测序分析，与伯氏疏螺旋体的法雷斯疏螺旋体相同，基因区间序列号分别为AB091447BvalaiOS115和DQ188933BvalaiQX13P25，相似率达100％。结论初步认为浙江省存在伯氏疏螺旋体病原。黄毛鼠、姬鼠、臭鼠句鼯可能为伯氏疏螺旋体的法雷斯疏螺旋体宿主，尚需进一步调查。

Detection and sequence analysis of Borrelia burgdorferi sensu lato DNA in rat-shape animals from Zhejiang province

Objective To investigate the natural infection situation of Borrelia burgdorferi sensu lato in rat-shape animals from Zhejiang province.Methods The segment between 5S rRNA and 23S rRNA of B.burgdorferi sensu lato in the rat-shape animals was amplified with the method of nest-PCR,and the positive PCR products were sequenced and analyzed.Results 128 rat-shape animals were captured in Jindong district of Jinhua city.12 positive samples were detected in Rattus,Apodemus and Shrew,and the positive rate were 9.68%,14.28% and 25.00%,respectively.The sequence analysis results showed the amplified segments from two positive samples were homologous to that of B.valaisiana and their gene serial numbers were AB091447BvalaiOS115 and DQ188933BvalaiQX13P25.The comparability reached 100% between them.Conclusion It suggested that there was B.burgdorferi sensu lato in Zhejiang province,and the rice field mouse,Apodemus and shrew could be the hosts of B.valaisiana.But it still needs be further studied.