应用定量蛋白质组学技术对IgA肾病肾组织蛋白的鉴定和定量分析

目的应用相对和绝对定量的同位素标记(iTRAQ)结合液相色谱和质谱技术,对IgA肾病肾组织蛋白质进行鉴定和定量分析。方法利用iTRAQ技术分析IgA肾病肾组织及正常对照肾组织中的蛋白质组信息,寻找显著差异表达蛋白质。结果共鉴定1 860个蛋白,其中287个蛋白在IgA肾病肾组织中显著上调表达,287个蛋白显著下调表达,最终筛选出9个明显上调蛋白和8个明显下调蛋白。结论定量蛋白质组学技术可有效地用于组织蛋白鉴定和相对定量,利于更好地了解蛋白质与IgA肾病发病机制的关系。

Differential proteomic analysis of renal tissues in patients with IgA nephropathy using quantitative proteomics technique

Objective To analyze and identify the protein of patients with IgA nephropathy for identification and quantitative a-nalysis using the combination of liquid chromatography and mass spectrometry which combined isobaric tags for relative and abso-lute quantification .Methods Proteomic analysis of renal tissue in patients with IgA nephropathy and normal controls was per-formed to identify different expressed proteins .Results A total of 1860 proteins were identified ,287 proteins were upregulated in renal tissue of IgA nephropathy patients ,287 proteins were downregulated significantly ,finally 9 upregulated proteins and 8 down regulated proteins were identified(protein fold difference greater than 1 .5) .Conclusion Quantitative proteomic technology is effi-ciently applicable for identification and relative quantitation of proteome in renal tissue ,which could get all the information of differ-ent protein .This is useful for us to better understand the relationship of protein and the pathogenesis of IGA nephropathy .